Protocol for the isolation and characterization of extracellular vesicles from human mesenchymal stromal cells.

Abigail Collins, Jennifer Liddle, Jeremy Balsbaugh, Joanne Walker, Heather Wanczyk, Christine Finck, Hala Saneh

Abstract read

In one paragraph

Article in STAR protocols, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it

0cells of the map it votes in

0citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Abigail CollinsDepartment of Pediatrics, University of Connecticut, Farmington, CT 06030, USA. Electronic address: abcollins@uchc.edu.

Jennifer LiddleProteomics and Metabolomics Facility, Center for Open Research Resources & Equipment, University of Connecticut, Storrs, CT 06269, USA.

Jeremy BalsbaughProteomics and Metabolomics Facility, Center for Open Research Resources & Equipment, University of Connecticut, Storrs, CT 06269, USA.

Joanne WalkerDepartment of Pediatrics, University of Connecticut, Farmington, CT 06030, USA.

Heather WanczykDepartment of Pediatrics, University of Connecticut, Farmington, CT 06030, USA.

Christine FinckDepartment of Pediatrics, University of Connecticut, Farmington, CT 06030, USA; Department of Pediatric Surgery, Connecticut Children's Medical Center, Hartford, CT 06106, USA.

Hala SanehDepartment of Pediatrics, University of Connecticut, Farmington, CT 06030, USA; Department of Neonatology, Connecticut Children's Medical Center, Hartford, CT 06106, USA. Electronic address: hsaneh@connecticutchildrens.org.

Funding

Thermo Scientific Orbitrap Eclipse Tribrid ETD Mass Spectrometer for UConn ProteomicsS10OD028445 · OD · UNIVERSITY OF CONNECTICUT STORRS · PI BALSBAUGH, JEREMY · 2021 to 2021

$994k

NIH HHS S10 OD028445

6 · The paper itself

Abstract

Here, we present a protocol for isolating extracellular vesicles (EVs) from the conditioned medium of human mesenchymal stromal cells (MSCs) and characterizing them using multiple modalities. We describe steps for enriching EVs by ultrafiltration and size-exclusion chromatography. We then detail procedures for imaging flow cytometry, transmission electron microscopy, nanoparticle tracking analysis, and the extraction of EV protein and RNA content. This workflow enables an integrated assessment of MSC-derived EVs to inform their use in diagnostic or therapeutic applications.

Indexed as

Cell cultureFlow CytometryProtein expression and purificationStem Cells

Identifiers

PMID42579556

DOI10.1016/j.xpro.2026.104745

PMCPMC13487827

What Socratic holds

Textmetadata

Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.