Evidence map›Paper›PMID 42579800›Full record

ArticleJCI insight2026

Mononuclear phagocyte-specific cGAS/STING targeting suppresses experimental choroidal neovascularization.

Le Shi, Durgadas Cherukaraveedu, Hongkwan Cho, Kaoru Ri, Lingli Zhou, Yingxue Cao, Narendra Kale, Zhenhua Xu, Wathsala Liyanage, Rangaramanujam M Kannan and 1 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in JCI insight, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Le ShiDepartment of Ophthalmology, School of Medicine, Johns Hopkins University, Baltimore, Maryland, USA.
Durgadas CherukaraveeduCenter for Nanomedicine at the Wilmer Eye Institute, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA.
Hongkwan ChoDepartment of Ophthalmology, School of Medicine, Johns Hopkins University, Baltimore, Maryland, USA.
Kaoru RiDepartment of Ophthalmology, School of Medicine, Johns Hopkins University, Baltimore, Maryland, USA.
Lingli ZhouDepartment of Ophthalmology, School of Medicine, Johns Hopkins University, Baltimore, Maryland, USA.
Yingxue CaoDepartment of Ophthalmology, School of Medicine, Johns Hopkins University, Baltimore, Maryland, USA.
Narendra KaleCenter for Nanomedicine at the Wilmer Eye Institute, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA.
Zhenhua XuDepartment of Ophthalmology, School of Medicine, Johns Hopkins University, Baltimore, Maryland, USA.
Wathsala LiyanageCenter for Nanomedicine at the Wilmer Eye Institute, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA.
Rangaramanujam M KannanCenter for Nanomedicine at the Wilmer Eye Institute, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA.
Elia J DuhDepartment of Ophthalmology, School of Medicine, Johns Hopkins University, Baltimore, Maryland, USA.

Funding

Wilmer Core Grant for Vision ResearchP30EY001765 · NEI · JOHNS HOPKINS UNIVERSITY · PI JEFFREY MUMM · 1985 to 2026
$22.1M
Soluble guanylate cyclase in diabetic retinopathyR01EY035549 · NEI · JOHNS HOPKINS UNIVERSITY · PI ELIA J DUH · 2024 to 2026
$1.4M
Reparative angiogenesis in ischemic retinopathyR01EY035897 · NEI · JOHNS HOPKINS UNIVERSITY · PI ELIA J DUH · 2024 to 2026
$1.2M
NEI NIH HHS P30 EY001765NEI NIH HHS R01 EY035549NEI NIH HHS R01 EY035897
6 · The paper itself

Abstract

Neovascular age-related macular degeneration (nAMD) is a major cause of blindness and is characterized by pathologic angiogenesis, specifically choroidal neovascularization (CNV). Mononuclear phagocytes (MPs), including infiltrating systemic monocyte-derived macrophages and retinal microglia, play critical roles in promoting CNV. The cGAS/STING pathway is increasingly implicated in multiple neuronal and systemic diseases and recently in ocular neovascularization. Given its roles across multiple cell types and the absence of MP-targeted therapies, we investigated the MP-specific role of cGAS/STING and a strategy for its selective targeting. In the laser-induced CNV mouse model, cGAS/STING was predominantly expressed in MPs. To enable selective targeting, we used a hydroxyl dendrimer (HD) previously shown to target MPs. HD conjugated to Cy3 selectively localized to MPs in laser CNV. HD conjugated to the STING inhibitor SN-011 (HD-SN-011) effectively inhibited cGAS/STING activation in cultured MPs. In the laser-CNV model, HD-SN-011 significantly reduced CNV leakage and lesion size, both important clinical endpoints in nAMD. RiboTag profiling confirmed selective suppression of cGAS/STING signaling and inflammatory gene expression in MPs. Together, our results implicate the specific importance of MP cGAS/STING signaling in CNV and provide proof of concept for specific modulation of STING in MPs as a therapy for nAMD.

Indexed as

Choroidal NeovascularizationMembrane ProteinsNucleotidyltransferasesAnimalscGAS-STING Signaling PathwayCyclic Guanosine Monophosphate-Adenosine Monophosphate SynthaseDisease Models, AnimalFemaleHumansMacrophagesMaleMiceMice, Inbred C57BLSTING ProteincGAS protein, mouseCyclic Guanosine Monophosphate-Adenosine Monophosphate SynthaseMembrane ProteinsNucleotidyltransferasesSting1 protein, mouseSTING ProteinDrug therapyInflammationMacrophagesOphthalmologyVascular biology

Identifiers

PMID42579800
PMCPMC13464012

What Socratic holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.