ArticleBiomedical chromatography : BMC2026
UV-Spectrophotometric and RP-HPLC Method Development and Validation for Simultaneous Estimation of Gallic Acid, Quercetin, Luteolin, and Apigenin in Saraca asoca Extract.
Article in Biomedical chromatography : BMC, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
0 citing papers in PubMed.
No citing paper in PubMed yet.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors.
Funding
Abstract
Phytochemicals have become the central focus of scientific research in recent times due to their vast applicability and comparatively low side effects. Gallic acid (GA), quercetin (QUE), luteolin (LUT), and apigenin (API) are natural antioxidants that are ubiquitous and co-exist in numerous medicinal plants and have been reported to have therapeutic potential in various diseases. The present study aims to develop and validate a simple, robust analytical method for the simultaneous estimation of these phytoconstituents in various plant extracts and pharmaceutical formulations. The UV and RP-HPLC methods were developed and validated in accordance with ICH Q2 guidelines. The UV method demonstrated good linearity and was found to be robust for the analysis of individual drugs, whereas the gradient HPLC method was suitable for the simultaneous estimation of these drugs. All method validation parameters were within acceptable limits; however, the HPLC method showed slight sensitivity to changes in method parameters, such as flow rate and wavelength. The developed UV spectrophotometric and gradient RP-HPLC methods for the simultaneous estimation of GA, QUE, LUT, and API were validated in accordance with the ICH guidelines. These analytical methods can facilitate simultaneous identification and quantification of phytoconstituents in crude extracts, pharmaceutical formulations, and biological samples.
Indexed as
Identifiers
What Socratic holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.