ArticleThe Journal of general virology2026
Rift Valley fever virus infects trophoblast cell lines in an LRP1-dependent manner.
Rachael E Rush, David A Price, Cynthia M McMillen, David L Donermeyer, Daisy W Leung, Gaya K Amarasinghe, Amy L Hartman
Abstract read
In one paragraphArticle in The Journal of general virology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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2 · The registryThe trial behind it
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4 · The recordCorrections and comments
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5 · Who and what moneyAuthors and funding
7 authors.
Rachael E RushCenter for Vaccine Research, School of Medicine, University of Pittsburgh, Pittsburgh, PA, USA.
David A PriceDepartment of Medicine, Washington University School of Medicine in St. Louis, St. Louis, MO, USA.
Cynthia M McMillenCenter for Vaccine Research, School of Medicine, University of Pittsburgh, Pittsburgh, PA, USA.
David L DonermeyerDepartment of Pathology and Immunology, Washington University School of Medicine in St. Louis, St. Louis, MO, USA.
Daisy W LeungDepartment of Medicine, Washington University School of Medicine in St. Louis, St. Louis, MO, USA.
Gaya K AmarasingheDepartment of Pathology and Immunology, Washington University School of Medicine in St. Louis, St. Louis, MO, USA.
Amy L HartmanCenter for Vaccine Research, School of Medicine, University of Pittsburgh, Pittsburgh, PA, USA.
Funding
Vaccines and Therapeutic Antibodies to Respiro, Rubula, Peribunya and Phenuiviridae (R2P2)-ReVAMPPU19AI181984 · NIAID · WASHINGTON UNIVERSITY · PI MOSCONA, ANNE, RAPPUOLI, RINO · 2024 to 2024
$44.1MPractices CoreUC7AI180311 · NIAID · UNIVERSITY OF PITTSBURGH AT PITTSBURGH · PI William Paul Duprex · 2023 to 2026
$13.2MViral Persistence and PathogenesisT32AI049820 · NIAID · UNIVERSITY OF PITTSBURGH AT PITTSBURGH · PI DELUCA, NEAL A. · 2001 to 2024
$5.5MIdentification and Characterization of Entry Factors Critical for Rift Valley Fever Virus Infection and PathogenesisR01AI161765 · NIAID · WASHINGTON UNIVERSITY · PI AMARASINGHE, GAYA K., HARTMAN, AMY L · 2021 to 2025
$3.7MLive-attenuated Rift Valley fever vaccines: comparative mechanisms of trans-placental transmission and vaccine efficacy for developing fetusesR01AI150792 · NIAID · UNIVERSITY OF PITTSBURGH AT PITTSBURGH · PI HARTMAN, AMY L · 2020 to 2025
$3.7MCharacterizing the role of LDL related receptor 1 (Lrp1) as host entry factor for multiple bunyavirusesR01AI169850 · NIAID · WASHINGTON UNIVERSITY · PI Gaya K. Amarasinghe, Amy L Hartman · 2023 to 2026
$3.1MHost and Viral Determinants of Orthobunyavirus Vertical Transmission: Novel Model Systems to Understand the Mechanisms of Congenital Disease in Humans and RuminantsK01AI165965 · NIAID · UNIVERSITY OF PITTSBURGH AT PITTSBURGH · PI Cynthia Marie McMillen · 2023 to 2026
$499kRole of Myeloid Cell Lrp1 in Rift Valley Fever Virus Infection and DisseminationF32AI191453 · NIAID · UNIVERSITY OF PITTSBURGH AT PITTSBURGH · PI WATKINS, RACHAEL RUSH · 2025 to 2025
$86kNIAID NIH HHS F32 AI191453NIAID NIH HHS K01 AI165965NIAID NIH HHS R01 AI150792NIAID NIH HHS R01 AI161765NIAID NIH HHS R01 AI169850NIAID NIH HHS T32 AI049820NIAID NIH HHS U19 AI181984NIAID NIH HHS UC7 AI180311
6 · The paper itselfAbstract
Rift Valley fever virus (RVFV) is a mosquito-borne phlebovirus that poses a growing global threat, causing severe disease in humans and livestock, including hepatitis, haemorrhage and encephalitis. Documented cases of vertical transmission in humans and livestock highlight risks during pregnancy. Although RVFV can directly infect the placenta, how it enters trophoblast cells remains unclear. Here, we investigated the role of low-density lipoprotein receptor-related protein 1 (LRP1), a known RVFV entry factor, in human trophoblast infection. Using JEG-3, JAR and HTR-8/SVneo trophoblast cell lines, we demonstrate that RVFV replicates to high titres and LRP1 expression varies across cell types. Competitive inhibition assays using the high-affinity LRP1 ligand murine RAP domain 3 and recombinant LRP1 fragments (cluster proteins) indicate that RVFV requires LRP1 for efficient trophoblast infection. Together, these findings implicate LRP1 as a determinant of RVFV infection at the maternal-fetal interface.
Indexed as
Low Density Lipoprotein Receptor-Related Protein-1Rift Valley FeverRift Valley fever virusTrophoblastsAnimalsCell LineFemaleHumansPlacentaPregnancyVirus InternalizationVirus ReplicationLow Density Lipoprotein Receptor-Related Protein-1LRP1 protein, humanbunyavirusCD91LRP1placentaRift Valley fevertrophoblast
Identifiers
PMID42584930
PMCPMC13465766
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