Enhanced polyphenolic production in tissue culture-derived Opuntia ficus-indica increases larvicidal activity against Spodoptera exigua: biochemical, histological and molecular docking evidence.

Enayat M Elqady, Eman El-Said, Moataz A M Moustafa, Reem Nasser Almozini, Naglaa Fathi Badr, Fatma S Ismail, Walaa H Ahmed, Marwa E Abd El-Sadek

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Article in Scientific reports, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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8 authors.

Enayat M ElqadyZoology and Entomology Department, Faculty of Science, Al-Azhar University, Naser City, Cairo, Egypt. EnayatElady.el.8.11@azhar.edu.eg.

Eman El-SaidZoology and Entomology Department, Faculty of Science, Al-Azhar University, Naser City, Cairo, Egypt.

Moataz A M MoustafaDepartment of Economic Entomology and Pesticides, Faculty of Agriculture, Cairo University, Giza, 12613, Egypt. moat_mon@agr.cu.edu.eg.

Reem Nasser AlmoziniDepartment of Biology, College of Science, Qassim University, Buraydah, Saudi Arabia.

Naglaa Fathi BadrZoology and Entomology Department, Faculty of Science, Al-Azhar University, Naser City, Cairo, Egypt.

Fatma S IsmailZoology and Entomology Department, Faculty of Science, Al-Azhar University, Naser City, Cairo, Egypt.

Walaa H AhmedZoology and Entomology Department, Faculty of Science, Al-Azhar University, Naser City, Cairo, Egypt.

Marwa E Abd El-SadekBotany and Microbiology Department, Faculty of Science, Al-Azhar University, Naser City, Cairo, Egypt.

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No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The accelerating resistance of Spodoptera exigua to synthetic insecticides demands the development of eco-friendly botanical alternatives. Plant tissue culture provides a controlled means to enhance the biosynthesis of defense metabolites. This study explored how in vitro micropropagation can enhance the polyphenolic profile and insecticidal efficacy of Opuntia ficus-indica cladode ethanolic extracts. These extracts, cultured on Murashige and Skoog medium without (Op1) or with plant growth regulators (Op2), were compared with greenhouse-grown (Op3) extracts for activity against second- and fourth-instar S. exigua larvae. Phytochemical analyses revealed differences among the extracts. The Op2 extract exhibited the highest total phenolic content (61.1 mg GAE g

Indexed as

InsecticidesOpuntiaPlant ExtractsPolyphenolsSpodopteraAnimalsLarvaMolecular Docking SimulationTissue Culture TechniquesInsecticidesPlant ExtractsPolyphenolsCladode extractsHPLC profilingMolecular dockingPolyphenolsTissue culture

Identifiers

PMID42587006

DOI10.1038/s41598-026-63384-4

PMCPMC13469097

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