Evidence mapPaperPMID 42587366Full record

ArticleChemMedChem2026

Tetramic Acid-Derived Blue-Emitting Fluorescent Probe for Selective Lipid Droplet Imaging in Live Cells and Zebrafish.

Marija Grozdanić, Jelena Dinić, Miloš Jović, Ana Podolski-Renić, Aleksandra Divac Rankov, Mila Ljujić, Milica Pešić, Igor M Opsenica, Života Selaković

Abstract read
In one paragraph

Article in ChemMedChem, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Marija GrozdanićInstitute for Biological Research "Siniša Stanković" - National Institute of the Republic of Serbia, University of Belgrade, Belgrade, Serbia.ORCID https://orcid.org/0009-0007-7425-685X
Jelena DinićInstitute for Biological Research "Siniša Stanković" - National Institute of the Republic of Serbia, University of Belgrade, Belgrade, Serbia.ORCID https://orcid.org/0000-0003-3371-2381
Miloš JovićInnovative Centre of the Faculty of Chemistry, University of Belgrade, Belgrade, Serbia.
Ana Podolski-RenićInstitute for Biological Research "Siniša Stanković" - National Institute of the Republic of Serbia, University of Belgrade, Belgrade, Serbia.ORCID https://orcid.org/0000-0001-7412-3685
Aleksandra Divac RankovInstitute of Molecular Genetics and Genetic Engineering, University of Belgrade, Belgrade, Serbia.ORCID https://orcid.org/0000-0001-6282-6746
Mila LjujićInstitute of Molecular Genetics and Genetic Engineering, University of Belgrade, Belgrade, Serbia.
Milica PešićInstitute for Biological Research "Siniša Stanković" - National Institute of the Republic of Serbia, University of Belgrade, Belgrade, Serbia.ORCID https://orcid.org/0000-0002-9045-8239
Igor M OpsenicaDepartment of Organic Chemistry, University of Belgrade - Faculty of Chemistry, Belgrade, Serbia.ORCID https://orcid.org/0000-0003-4942-4042
Života SelakovićDepartment of Organic Chemistry, University of Belgrade - Faculty of Chemistry, Belgrade, Serbia.ORCID https://orcid.org/0000-0003-0322-6884

Funding

Ministry of Science, Technological Development, and Innovation of the Republic of Serbia 451-03-33/2026-03/200007Ministry of Science, Technological Development, and Innovation of the Republic of Serbia 451-03-33/2026-03/200042Ministry of Science, Technological Development, and Innovation of the Republic of Serbia 451-03-33/2026-03/200168Ministry of Science, Technological Development, and Innovation of the Republic of Serbia 451-03-33/2026-03/200288
6 · The paper itself

Abstract

Lipid droplets (LDs) are dynamic intracellular organelles involved in lipid storage and metabolism, and their imaging is essential for studying metabolic processes in health and disease. Here, we describe four novel fluorescent tetramic acid derivatives as potential LD markers for live imaging in cellular and whole-organism systems, and designate the compound with the most favorable properties, 1a, for further characterization. The probe 1a was evaluated in multiple cell lines, where it enabled clear visualization of intracellular LDs and showed strong colocalization with established lipid markers such as Nile Red and BODIPY 493/503. Photophysical characterization revealed photostability and signal-to-noise characteristics enabling fluorescence imaging applications. In addition, cytotoxicity assays indicated minimal acute and delayed effects at imaging concentrations. Importantly, 1a was successfully applied in a zebrafish model, enabling visualization of lipid-rich structures in a whole-organism context. Together, these findings identify 1a as a useful LD fluorescent marker for live imaging applications both in vitro and in vivo.

Indexed as

Fluorescent DyesLipid DropletsOptical ImagingAnimalsFluorescent Chemosensor CompoundsHumansMolecular StructurePyrrolidinonesZebrafishFluorescent Chemosensor CompoundsFluorescent DyesPyrrolidinonestetramic acidblue‐emitting probefluorescent probelipid dropletslive‐cell imagingzebrafish model

Identifiers

PMID42587366
PMCPMC13469721

What Socratic holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.