ArticleMolecules (Basel, Switzerland)2026
Lipidomics Based on HILIC-ESI-HRMS Reveal Subtle Changes in the Mitochondrial Phospholipidome of Mouse Embryonic Fibroblasts Expressing Pathogenic Variants of Human OPA1 Protein.
Andrea Castellaneta, Ilario Losito, Vito Porcelli, Serena Barile, Alessandra Maresca, Valentina Del Dotto, Penelope Magnoni, Claudia Zanna, Ludovica Sofia Guadalupi, Valeria Cinquepalmi and 5 more
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In one paragraphArticle in Molecules (Basel, Switzerland), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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5 · Who and what moneyAuthors and funding
15 authors.
Andrea CastellanetaDipartimento di Chimica, Università degli Studi di Bari Aldo Moro, Via Orabona 4, 70126 Bari, Italy.ORCID 0000-0001-5249-7168 Ilario LositoDipartimento di Chimica, Università degli Studi di Bari Aldo Moro, Via Orabona 4, 70126 Bari, Italy.ORCID 0000-0003-0025-3350 Vito PorcelliDipartimento di Bioscienze, Biotecnologie e Ambiente, Università degli Studi di Bari Aldo Moro, Via Orabona 4, 70126 Bari, Italy.ORCID 0000-0002-3634-9613 Serena BarileDipartimento di Bioscienze, Biotecnologie e Ambiente, Università degli Studi di Bari Aldo Moro, Via Orabona 4, 70126 Bari, Italy.
Alessandra MarescaProgramma di Neurogenetica, IRCCS Istituto delle Scienze Neurologiche di Bologna, Via Altura 3, 40139 Bologna, Italy.ORCID 0000-0002-8959-8832 Valentina Del DottoDipartimento di Scienze Biomediche e Neuromotorie, Università degli Studi di Bologna, Via Altura 3, 40139 Bologna, Italy.ORCID 0000-0002-6053-0893 Penelope MagnoniProgramma di Neurogenetica, IRCCS Istituto delle Scienze Neurologiche di Bologna, Via Altura 3, 40139 Bologna, Italy.
Claudia ZannaDipartimento di Scienze Biomediche e Neuromotorie, Università degli Studi di Bologna, Via Altura 3, 40139 Bologna, Italy.ORCID 0000-0002-0647-5360 Ludovica Sofia GuadalupiDipartimento di Chimica, Università degli Studi di Bari Aldo Moro, Via Orabona 4, 70126 Bari, Italy.ORCID 0009-0001-1168-5733 Valeria CinquepalmiDipartimento di Chimica, Università degli Studi di Bari Aldo Moro, Via Orabona 4, 70126 Bari, Italy.ORCID 0009-0002-0733-4452 Cosima Damiana CalvanoDipartimento di Chimica, Università degli Studi di Bari Aldo Moro, Via Orabona 4, 70126 Bari, Italy.ORCID 0000-0001-8832-7072 David C ChanDivision of Biology and Biological Engineering, California Institute of Technology, 1200 East California Boulevard, Pasadena, CA 91125, USA.
Valerio CarelliProgramma di Neurogenetica, IRCCS Istituto delle Scienze Neurologiche di Bologna, Via Altura 3, 40139 Bologna, Italy.ORCID 0000-0003-4923-6404 Luigi PalmieriDipartimento di Bioscienze, Biotecnologie e Ambiente, Università degli Studi di Bari Aldo Moro, Via Orabona 4, 70126 Bari, Italy.ORCID 0000-0002-6503-2922 Tommaso R I CataldiDipartimento di Chimica, Università degli Studi di Bari Aldo Moro, Via Orabona 4, 70126 Bari, Italy.ORCID 0000-0003-4811-8959 Funding
Ministry of Universities and Research 20172T2MHH_002
6 · The paper itselfAbstract
Pathogenic variants of the mammalian optic atrophy 1 (OPA1) protein, a key regulator of mitochondrial fusion, may lead to severe mitochondrial dysfunction and morphological alterations, and have also been proposed to be involved in remodeling the mitochondrial membranes lipid profile. To address this last issue, a lipidomic workflow based on HILIC-ESI-HRMS was applied to profile major classes of mitochondrial membrane phospholipids (PL), namely phosphatidylcholines (PCs), -ethanolamines (PEs), and -inositols (PIs), along with cardiolipins (CLs), in mouse embryonic fibroblasts knocked out for Opa1 gene (Opa1
Indexed as
FibroblastsGTP PhosphohydrolasesLipidomicsMitochondriaPhospholipidsAnimalsEmbryo, MammalianHumansLiquid Chromatography-Mass SpectrometryMiceMitochondrial MembranesSpectrometry, Mass, Electrospray IonizationGTP PhosphohydrolasesOPA1 protein, humanOpa1 protein, mousePhospholipidsHILIC-ESI-HRMShuman OPA1lipidomicsmitochondriamouse embryonic fibroblastsOPA1 variants
Identifiers
PMID42588565
PMCPMC13468052
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