Bioactive Peptide Fractions from Mung Bean Protein Hydrolysate: Antioxidant Activity, Anti-Inflammatory Effects, and Immunomodulatory Responses in RAW 264.7 Macrophages.

Worrapob Chaisan, Kanokwan Promjeen, Komgrit Eawsakul, Chawan Manaspon, Niramon Utama-Ang

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Article in International journal of molecular sciences, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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5 · Who and what money

Authors and funding

5 authors.

Worrapob ChaisanCluster of High Value Products from Thai Rice and Plants for Health, Chiang Mai University, Chiang Mai 50100, Thailand.ORCID 0009-0002-0952-9608

Kanokwan PromjeenDivision of Product Development Technology, Faculty of Agro Industry, Chiang Mai University, Chiang Mai 50100, Thailand.ORCID 0009-0003-6865-2582

Komgrit EawsakulApplied Thai Traditional Medicine, School of Medicine, Walailak University, Nakhon Si Thammarat 80160, Thailand.

Chawan ManasponBiomedical Engineering Institute, Chiang Mai University, Chiang Mai 50100, Thailand.ORCID 0000-0001-8961-7901

Niramon Utama-AngCluster of High Value Products from Thai Rice and Plants for Health, Chiang Mai University, Chiang Mai 50100, Thailand.

Funding

Chiang Mai University Fundamental Fund 2025 (207635)Thailand Science Research and Innovation FRB680102/0162

6 · The paper itself

Abstract

Amid growing concerns over climate change and the need for sustainable agriculture, the study investigated the antioxidant, anti-inflammatory, and immunomodulatory activities of MBPH fractions. MBPH was produced via alcalase hydrolysis and fractionated by ultrafiltration into >5, 3-5, 1-3, and <1 kDa fractions. Bioactivity exhibited a clear inverse correlation with molecular weight. The <1 and 1-3 kDa fractions demonstrated their highest antioxidant capabilities through their performance in ORAC and ABTS tests, which produced results of 836.51 ± 11.81 µmol TE/g and 19.36 ± 0.71 µmol TE/g, respectively. Both fractions also demonstrated significant in vitro anti-inflammatory potential, with the <1 kDa fraction showing superior activity by inhibiting LOX activity (76.93 ± 1.53%) and heat-induced albumin denaturation (81.82 ± 2.72%). All fractions were non-cytotoxic, preserving >90% cell viability in RAW 264.7 macrophages at 100 µg/mL. The 1-3 kDa MBPH fraction exhibited distinct context-dependent immunomodulatory activity in RAW 264.7 macrophages. At 3 h, the fraction markedly suppressed

Indexed as

Anti-Inflammatory AgentsAntioxidantsImmunologic FactorsMacrophagesPeptidesProtein HydrolysatesVignaAnimalsBioactive Peptides, DietaryCell SurvivalLipopolysaccharidesMiceRAW 264.7 CellsTumor Necrosis Factor-alphaAnti-Inflammatory AgentsAntioxidantsBioactive Peptides, DietaryImmunologic FactorsLipopolysaccharidesPeptidesProtein HydrolysatesTumor Necrosis Factor-alphaenzyme inhibitionimmunomodulationoxidative stressplant-derived peptidessize-based fractionationVigna radiata L.

Identifiers

PMID42589538

DOI10.3390/ijms27156882

PMCPMC13466527

What Socratic holds

Textmetadata

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.