ArticleSTAR protocols2026
Protocol for assisted sperm fusion insemination of human oocytes using zona pellucida-bound sperm.
Article in STAR protocols, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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4 authors.
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Abstract
Oocyte degeneration is a critical and persistent limitation of intracytoplasmic sperm injection (ICSI). Here, we present assisted sperm fusion insemination (ASFI), a protocol for fertilizing human oocytes using zona pellucida-bound sperm (ZP-sperm) to minimize oocyte degeneration while preserving cellular integrity. We describe steps for co-culturing immature or degenerated oocytes with sperm, harvesting ZP-sperm, and facilitating their attachment to and fusion with oocytes. This protocol uses standard ICSI equipment and provides a reproducible approach for minimizing the mechanical stress during fertilization. For complete details on the use and execution of this protocol, please refer to Hatakeyama et al.
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