ArticleFrontiers in nutrition2026
RSM and GA-BP assisted optimization of enzyme-ultrasound assisted extraction for mulberry fruit polysaccharides and comparison of their protective effects on NAFLD with hot water extraction polysaccharide.
Article in Frontiers in nutrition, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
0 citing papers in PubMed.
No citing paper in PubMed yet.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Background: Non-alcoholic fatty liver disease (NAFLD) is triggered by gut-liver axis disorders; enzyme-ultrasound assisted extraction (EAUE) better retains bioactive mulberry polysaccharides (MP) than hot water extraction. This work optimized EAUE by RSM and GA-BP to compare the gut-hepatic protective capacity of two MP preparations and reveal their microecological regulatory mechanism. Methods: We optimized EAUE conditions and characterized the structural features of EAUE-derived MP (MPEU). A 30-day concurrent modeling and MP-administration mouse trial was conducted with biochemical detection and 16S rRNA sequencing to assess MPEU and hot water extraction MP (MPHW) Results: RSM yielded predictions closer to experimental values, and its optimal extraction conditions were determined as extraction time 107 min, liquid-to-solid ratio 33:1, enzyme addition amount 350 mg, and extraction temperature 52 °C, yielding MP at 10.06 ± 0.03%. MPEU retained complete glycosidic structures and outperformed MPHW in mitigating hepatic steatosis, intestinal damage and inflammation, by restoring SCFA-producing commensal bacteria and suppressing pro-inflammatory flora. Conclusion: In this study, RSM is preferable for EAUE optimization. Structurally intact MPEU exerts stronger NAFLD relief via remodeling gut microecology, promising as nutritional functional food ingredients.
Indexed as
Identifiers
What Socratic holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.