Evidence map›Paper›PMID 42603245›Full record

ArticleStem cell reviews and reports2026

Extracellular Vesicles Derived from Resveratrol-Treated BMSCs Promote the Repair of Bone Defects in Osteoporotic Rats.

Yan Lv, Yongjun Du, Zhi Peng, Chengjie Ma, Zhenggang Li, Rui Xu, Jianhong Hou, Sheng Lu

Abstract read
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In one paragraph

Article in Stem cell reviews and reports, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Yan Lv *Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming, China.
Yongjun Du *Department of Orthopedics, the Key Laboratory of Digital Orthopedics of Yunnan Province, the Clinical Medicine Center of Spinal and Spinal Cord Disorders of Yunnan Province, the First People's Hospital of Yunnan Province & the Affiliated Hospital of Kunming University of Science and Technology, Kunming, China.
Zhi PengDepartment of Orthopedics, the Key Laboratory of Digital Orthopedics of Yunnan Province, the Clinical Medicine Center of Spinal and Spinal Cord Disorders of Yunnan Province, the First People's Hospital of Yunnan Province & the Affiliated Hospital of Kunming University of Science and Technology, Kunming, China.
Chengjie MaDepartment of Orthopedics, the Key Laboratory of Digital Orthopedics of Yunnan Province, the Clinical Medicine Center of Spinal and Spinal Cord Disorders of Yunnan Province, the First People's Hospital of Yunnan Province & the Affiliated Hospital of Kunming University of Science and Technology, Kunming, China.
Zhenggang LiFaculty of Life Science and Technology, Kunming University of Science and Technology, Kunming, China.
Rui XuFaculty of Life Science and Technology, Kunming University of Science and Technology, Kunming, China.
Jianhong HouDepartment of Orthopedics, the Key Laboratory of Digital Orthopedics of Yunnan Province, the Clinical Medicine Center of Spinal and Spinal Cord Disorders of Yunnan Province, the First People's Hospital of Yunnan Province & the Affiliated Hospital of Kunming University of Science and Technology, Kunming, China. 18808713303@163.com.
Sheng LuDepartment of Orthopedics, the Key Laboratory of Digital Orthopedics of Yunnan Province, the Clinical Medicine Center of Spinal and Spinal Cord Disorders of Yunnan Province, the First People's Hospital of Yunnan Province & the Affiliated Hospital of Kunming University of Science and Technology, Kunming, China. lusheng@kust.edu.cn.

Funding

Major Project under the Open Research Grant of Yunnan Provincial Clinical Medical Center for Spinal and Spinal Cord Diseases 2024YNLCYXZX0247National Natural Science Foundation of China 82172442"YunLing Scholars" Program of Yunnan Province XDYC-YLXZ-2022-0015Yunnan Provincial Health Commission Medical Reserve Talents Project H2024032
6 · The paper itself

Abstract

Osteoporotic fracture is the most severe complication of osteoporosis, and impaired bone regeneration and delayed healing after fracture remain major clinical challenges. Extracellular vesicles (EVs) derived from bone marrow mesenchymal stem cells (BMSCs) may play a critical role in promoting bone regeneration after osteoporotic fracture. Enhancing their osteogenic potential and elucidating the underlying mechanisms may contribute to accelerated fracture healing. In this study, resveratrol-stimulated BMSC-derived EVs (Res-EVs) were isolated using differential centrifugation combined with ultracentrifugation. We found that compared with EVs secreted by BMSCs under normal culture conditions (Nor-EVs), Res-EVs significantly enhanced BMSCs osteogenic differentiation capacity. Analysis of miRNA sequencing data identified miR-19a-3p as significantly downregulated and PC-3p-83,608 as significantly upregulated. The reliability of the sequencing data was validated by qPCR results. By transfecting BMSCs with inhibitor and mimic of miR-19a-3p, we found that miR-19a-3p was a negative regulator of osteogenic differentiation. Bioinformatics prediction and dual-luciferase reporter assays confirmed that miR-19a-3p directly targets Raf1. Western blot results indicated that miR-19a-3p regulates osteogenic differentiation of BMSCs by targeting Raf1. In vivo experiments further demonstrated that Res-EVs treatment enhanced repair at osteoporotic fracture sites and increased the expression of RUNX2 and RAF1. Collectively, these findings indicate that Res-EVs promote bone regeneration at osteoporotic bone defect sites in rats, with the miR-19a-3p/Raf1 axis representing a potential underlying mechanism.

Indexed as

Extracellular vesiclesmiRNAOsteogenic differentiationOsteoporotic fractureRaf1

Identifiers

PMID42603245

What Socratic holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.