Ultrasound-assisted deep eutectic solvent extraction and macroporous resin enrichment of polyphenols from Cortex Mori: Screening of tyrosinase inhibitors via affinity ultrafiltration-LC-MS and molecular docking.

Wei Dai, Manqiu Lei, Sina Wang, Lei Jin, Haijun Nan, Liming Pan

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Article in Ultrasonics sonochemistry, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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6 authors.

Wei DaiTeaching and Experimental Center, Guangdong Pharmaceutical University, Guangzhou 510006, China; Comprehensive Experimental Teaching Center of Traditional Chinese Medicine, Yunfu Campus, Guangdong Pharmaceutical University, Yunfu 527500, China. Electronic address: dai_gdpu_2018@gdpu.edu.cn.

Manqiu LeiSchool of Traditional Chinese Materia Medica, Guangdong Pharmaceutical University, Guangzhou 510006, China.

Sina WangTeaching and Experimental Center, Guangdong Pharmaceutical University, Guangzhou 510006, China; Comprehensive Experimental Teaching Center of Traditional Chinese Medicine, Yunfu Campus, Guangdong Pharmaceutical University, Yunfu 527500, China.

Lei JinTeaching and Experimental Center, Guangdong Pharmaceutical University, Guangzhou 510006, China; Comprehensive Experimental Teaching Center of Traditional Chinese Medicine, Yunfu Campus, Guangdong Pharmaceutical University, Yunfu 527500, China.

Haijun NanSchool of Traditional Chinese Materia Medica, Guangdong Pharmaceutical University, Guangzhou 510006, China. Electronic address: nanhaijun@gdpu.edu.cn.

Liming PanSchool of Traditional Chinese Materia Medica, Guangdong Pharmaceutical University, Guangzhou 510006, China. Electronic address: panliming@gdpu.edu.cn.

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6 · The paper itself

Abstract

Cortex Mori, the dried root bark of Morus alba L., is a rich source of polyphenolic compounds. In this study, an ultrasound-assisted deep eutectic solvent (UAE-DES) strategy was developed for the efficient extraction of polyphenols from Cortex Mori, combined with macroporous resin enrichment and subsequent chemical and functional characterization. Among 36 deep eutectic solvents evaluated, betaine-sucrose (DES-6) exhibited the best extraction performance. Response surface methodology optimized the UAE-DES conditions as 35% water content, 57 °C, liquid-to-solid ratio of 90 mL/g, ultrasound time of 20 min, and power of 520 W, achieving a total phenolic yield of 232.93 ± 0.95 mg GAE/g, which was 28.31% and 32.78% higher than the highest yields obtained using methanol and ethanol, respectively. Scanning electron microscopy revealed that ultrasound-DES treatment caused pronounced erosion, pore formation, cracking, and partial collapse of plant cell walls, thereby facilitating solvent penetration and mass transfer. After purification using HPD-100 resin, UHPLC-Q-Orbitrap HRMS identified 58 polyphenolic compounds, including 11 reported from Cortex Mori for the first time. The 70-W2 fraction exhibited strong tyrosinase inhibitory activity (IC

Indexed as

Chemical FractionationDeep Eutectic SolventsEnzyme InhibitorsMolecular Docking SimulationMonophenol MonooxygenaseMorusPolyphenolsResins, SyntheticUltrasonic WavesLiquid Chromatography-Mass SpectrometryPorosityUltrafiltrationDeep Eutectic SolventsEnzyme InhibitorsMonophenol MonooxygenasePolyphenolsResins, SyntheticAffinity ultrafiltration‑LC‑MSCortex MoriDeep eutectic solventMacroporous resin fractionationMolecular dockingPolyphenolsTyrosinase inhibitionUltrasound‑assisted extraction

Identifiers

PMID42604606

DOI10.1016/j.ultsonch.2026.108006

PMCPMC13499495

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