Evidence map›Paper›PMID 42618629›Full record

ArticleNature chemical biology2026

A chemigenetic endogenous Ras activity biosensor for live-cell pharmacology.

Ryan Weeks, Daojia R Zhou, Michelle S Frei, Jin Zhang

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Nature chemical biology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Ryan WeeksDepartment of Pharmacology, University of California, San Diego, La Jolla, CA, USA.
Daojia R ZhouDepartment of Pharmacology, University of California, San Diego, La Jolla, CA, USA.
Michelle S FreiDepartment of Pharmacology, University of California, San Diego, La Jolla, CA, USA.ORCID http://orcid.org/0000-0002-4799-4554
Jin ZhangDepartment of Pharmacology, University of California, San Diego, La Jolla, CA, USA. jzhang32@health.ucsd.edu.ORCID http://orcid.org/0000-0001-7145-7823

Funding

Live-cell Activity Architecture in CancerR35CA197622 · NCI · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI Jin Zhang · 2015 to 2026
$11.2M
U of Calif, San Diego Neuroscience Microscopy ImagingP30NS047101 · NINDS · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI GLEESON, JOSEPH G, ZHENG, BINHAI · 2003 to 2022
$9.0M
NCI NIH HHS R35 CA197622NINDS NIH HHS P30 NS047101Schweizerischer Nationalfonds zur Förderung der Wissenschaftlichen Forschung (Swiss National Science Foundation) P500PN_214234U.S. Department of Health & Human Services | NIH | Office of Extramural Research, National Institutes of Health (OER) NS047101
6 · The paper itself

Abstract

Ras is a small GTPase that regulates cell growth and proliferation. Hyperactive Ras is prevalent in cancer and has been a therapeutic target for decades. Many lines of evidence have demonstrated that Ras signals from the plasma membrane as well as noncanonical compartments such as the Golgi, making live-cell biosensors for tracking the spatiotemporal dynamics of Ras activity a valuable approach for Ras studies. However, current biosensors are limited in their quantitative capacity for measuring endogenous Ras activity. Here we introduce a chemigenetic biosensor design, making use of circularly permuted HaloTag labeled with the fluorophore JF

Indexed as

Biosensing Techniquesras ProteinsChemogeneticsFluorescent Chemosensor CompoundsHumansSignal TransductionFluorescent Chemosensor Compoundsras Proteins

Identifiers

PMID42618629
PMCPMC13618761

What Socratic holds

Textmetadata
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.