Evidence map›Paper›PMID 42649428›Full record

ArticleNature structural & molecular biology2026

Characterizing dynamics of productive dynein stepping by MINFLUX.

Joseph Slivka, Emma S Gleave-Hanford, Mert Golcuk, Devinda P Wijewardena, John T Canty, Paul R Selvin, Mert Gur, Andrew P Carter, Ahmet Yildiz

Abstract read
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Article in Nature structural & molecular biology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Joseph SlivkaDepartment of Physics, University of California at Berkeley, Berkeley, CA, USA.
Emma S Gleave-HanfordMedical Research Council Laboratory of Molecular Biology, Division of Structural Studies, Francis Crick Avenue, Cambridge, UK.
Mert GolcukDepartment of Computational and Systems Biology, University of Pittsburgh, Pittsburgh, PA, USA.ORCID http://orcid.org/0000-0001-5476-8160
Devinda P WijewardenaDepartment of Chemical and Biomolecular Engineering, University of Illinois at Urbana-Champaign, Urbana, IL, USA.
John T CantyBiophysics Graduate Group, University of California at Berkeley, Berkeley, CA, USA.
Paul R SelvinDepartment of Physics, University of Illinois at Urbana-Champaign, Urbana, IL, USA.ORCID http://orcid.org/0000-0002-3658-4218
Mert GurDepartment of Computational and Systems Biology, University of Pittsburgh, Pittsburgh, PA, USA.ORCID http://orcid.org/0000-0003-0983-4397
Andrew P CarterMedical Research Council Laboratory of Molecular Biology, Division of Structural Studies, Francis Crick Avenue, Cambridge, UK.ORCID http://orcid.org/0000-0001-7292-5430
Ahmet YildizDepartment of Physics, University of California at Berkeley, Berkeley, CA, USA. yildiz@berkeley.edu.ORCID http://orcid.org/0000-0003-4792-174X

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Cytoplasmic dynein drives minus-end-directed motility along microtubules by converting ATP hydrolysis into coordinated structural changes but how this process produces directional stepping remains unresolved. The dynamics of dynein stepping have previously been characterized by tracking flexible regions of the motor with limited resolution. Here we site-specifically labeled the microtubule-binding domain of yeast dynein using a cysteine-light mutant and tracked stepping with submillisecond, nanometer precision at physiological ATP using minimal fluorescence photon flux (MINFLUX). We show that dynein hydrolyzes one ATP per step and moves in multiples of 8 nm. Steps are preceded by a transient plus-end-directed displacement ('dip'), corresponding to microtubule release upon ATP binding and diffusion of the stepping head around its partner. A slow ATP-hydrolyzing mutant shows more frequent dips, supporting a model in which ATP hydrolysis produces net forward movement. These results clarify the sequence of mechanochemical events underlying productive dynein stepping.

Indexed as

DyneinsSaccharomyces cerevisiaeSaccharomyces cerevisiae ProteinsAdenosine TriphosphateHydrolysisMicrotubulesAdenosine TriphosphateDyneinsSaccharomyces cerevisiae Proteins

Identifiers

PMID42649428

What Socratic holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.