Evidence map›Paper›PMID 42650792›Full record

ArticleBiomolecules2026

Construction of Engineered

Haoni Luan, Rui Yang, Wenhan Qiu, Kaiyue Feng, Wei Xu, Fei Wang, Wei Feng, Peng Song

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biomolecules, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Haoni LuanSchool of Pharmaceutical Sciences and Food Engineering, Liaocheng University, Liaocheng 252000, China.
Rui YangSchool of Pharmaceutical Sciences and Food Engineering, Liaocheng University, Liaocheng 252000, China.
Wenhan QiuSchool of Pharmaceutical Sciences and Food Engineering, Liaocheng University, Liaocheng 252000, China.
Kaiyue FengCollege of Agriculture and Biology, Liaocheng University, Liaocheng 252000, China.
Wei XuSchool of Pharmaceutical Sciences and Food Engineering, Liaocheng University, Liaocheng 252000, China.
Fei WangSchool of Pharmaceutical Sciences and Food Engineering, Liaocheng University, Liaocheng 252000, China.
Wei FengSchool of Pharmaceutical Sciences and Food Engineering, Liaocheng University, Liaocheng 252000, China.
Peng SongSchool of Pharmaceutical Sciences and Food Engineering, Liaocheng University, Liaocheng 252000, China.ORCID 0000-0002-9195-7769

Funding

National Natural Science Foundation of China No. 82304838Natural Science Foundation of Shandong Province ZR2022MC159
6 · The paper itself

Abstract

Carcinine is an imidazole dipeptide with potent antioxidant and antiglycation properties, although its chemical synthesis currently relies on severely environmentally harmful processes. In this work, a multi-enzyme cascade biotransformation system comprising 4'-phosphopantetheinyl transferase and non-ribosomal peptide synthetase was constructed. To overcome the limitations arising from stochastic spatial distribution and suboptimal mass transfer associated with independent enzymes, a fusion protein strategy was adopted. The two enzymes were fused via a flexible genetic linker within plasmid pET28a-SFP-L-Ebony, which enabled robust soluble expression in

Indexed as

Aminoimidazole CarboxamideEscherichia coliPeptide SynthasesBacterial ProteinsBiocatalysisCRISPR-Cas SystemsTransferases (Other Substituted Phosphate Groups)Aminoimidazole CarboxamideBacterial Proteinsnon-ribosomal peptide synthasePeptide Synthasesphosphopantetheinyl transferaseTransferases (Other Substituted Phosphate Groups)biosynthesiscarcinineCRISPR/Cas9fusion proteinmulti-enzyme cascade catalysis

Identifiers

PMID42650792
PMCPMC13510768

What Socratic holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.