Evidence map›Paper›PMID 42654752›Full record

ArticlePathogens (Basel, Switzerland)2026

Performance of High-Resolution Melting Analysis for Molecular Detection and Species Differentiation of Malaria: A Scoping Review.

Afiat Berbudi, Shafia Khairani, Andromeda Andromeda, Alexander Kwarteng

Abstract readScoping Review
In one paragraph

Article in Pathogens (Basel, Switzerland), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Afiat BerbudiDepartment of Biomedical Sciences, Parasitology Division, Faculty of Medicine, Universitas Padjadjaran, Bandung 40161, Indonesia.
Shafia KhairaniDepartment of Biomedical Sciences, Cell Biology Division, Faculty of Medicine, Universitas Padjadjaran, Bandung 40161, Indonesia.ORCID 0000-0002-6225-1388
Andromeda AndromedaDepartement of Biomedical Sciences, Parasitology Division, Faculty of Medicine, Pasundan University, Bandung 40117, Indonesia.
Alexander KwartengDepartment of Biochemistry and Biotechnology, Kwame Nkrumah University of Science and Technology, PMB University Post Office, Kumasi, Ghana.

Funding

Indonesia Endowment Fund for Education 4303/B3/DT.03.08/2025 and 3927/UN6.RKT/HK.07.00/2025.
6 · The paper itself

Abstract

Malaria diagnosis remains challenging in low-density, asymptomatic, mixed-species, and non-falciparum infections. This scoping review mapped the diagnostic and operational evidence for high-resolution melting (HRM) analysis in malaria. Following PRISMA-ScR guidance, PubMed and Scopus were searched for English-language studies published between 2016 and 2026. Two reviewers independently screened records in Rayyan. Of 162 records identified, 15 studies met the eligibility criteria. Most assays targeted 18S rRNA, although mitochondrial, apicoplast, species-specific, and resistance-associated markers were also evaluated. Reported sensitivity ranged from 91.76% to 100%, and specificity generally exceeded 92%. HRM enabled differentiation of major human-infecting

Indexed as

MalariaMolecular Diagnostic TechniquesPlasmodiumHumansRNA, Ribosomal, 18SSensitivity and SpecificityRNA, Ribosomal, 18Shigh-resolution meltingHRMmalariamolecular diagnosisPlasmodium

Identifiers

PMID42654752
PMCPMC13516130

What Socratic holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.