Evidence map›Paper›PMID 42655813›Full record

ArticleVeterinary sciences2026

Establishment of an Indirect ELISA Detection Method for Porcine Reproductive and Respiratory Syndrome Virus NSP10.

Gan Li, Qipeng Zhang, Xiaojing Chen, Huawei Li, Ruining Wang, Keshan Zhang, Yaqiong Ye, Mengmeng Zhao

Abstract read
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In one paragraph

Article in Veterinary sciences, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0cells of the map it votes in
0citing papers in PubMed
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

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5 · Who and what money

Authors and funding

8 authors.

Gan LiSchool of Animal Science and Technology, Foshan University, Foshan 528225, China.
Qipeng ZhangSchool of Animal Science and Technology, Foshan University, Foshan 528225, China.
Xiaojing ChenSchool of Animal Science and Technology, Foshan University, Foshan 528225, China.
Huawei LiInstitute of Animal Product Quality and Safety Technology, Henan University of Animal Husbandry and Economy, Zhengzhou 450046, China.
Ruining WangInstitute of Animal Product Quality and Safety Technology, Henan University of Animal Husbandry and Economy, Zhengzhou 450046, China.
Keshan ZhangSchool of Animal Science and Technology, Foshan University, Foshan 528225, China.ORCID 0009-0002-0306-0327
Yaqiong YeSchool of Animal Science and Technology, Foshan University, Foshan 528225, China.
Mengmeng ZhaoSchool of Animal Science and Technology, Foshan University, Foshan 528225, China.ORCID 0000-0003-4143-3606

Funding

Base and Talent Program of Science and Technology Plan in Tibet Autonomous Region XZ202401JD0012Foshan University High Level Talents Research Start-up Project CGZ07465/CGZ07001Guangdong Basic and Applied Basic Research Foundation 2024A1515140152Guangdong Provincial Department of Education's distinctive innovation initiative 2023KTSCX128Key Research and Development Special Project of Henan Province 251111113600Key Scientific and Technological Grants in Henan Province 24210211031National Key Research and Development Program Projects 2023YFD1801302, 2023YFD1801301National Natural Science Foundation of China 31902279Open Foundation of Guangdong Provincial Key Laboratory of Animal Molecular Design and Pre-cise Breeding 2024B03Special Funds for Rural Revitalization Strategy of Guangdong Provincial Department of Agriculture and Rural Affairs in 2024 2024CXTD15
6 · The paper itself

Abstract

As a highly contagious infectious disease in the swine industry, porcine reproductive and respiratory syndrome (PRRS) can cause reproductive disorders in sows and respiratory diseases in piglets, resulting in sustained economic losses. Nonstructural protein 10 (NSP 10), as one of the core components of the replication and transcription complex of PRRS virus (PRRSV), has both helicase and adenosine triphosphatase activities, and has the potential as a diagnostic marker. In this study, NSP10 recombinant protein was used as coating antigen, and an indirect ELISA method for PRRSV NSP10 detection was established by square matrix titration. After optimizing the assay parameters, high specificity (no reactions with other pig viruses), high sensitivity (able to detect antibodies even when the positive blood sample was diluted 1280 times), and excellent reproducibility (less than 10% variation) were shown. Therefore, the NSP10 indirect enzyme-linked immunosorbent assay (ELISA) developed in this study provides an effective technical means for enhancing PRRSV surveillance in swine herds, thereby establishing a solid foundation for the clinical diagnosis and comprehensive prevention and control of PRRSV.

Indexed as

detection methodenzyme-linked immunosorbent assayNSP10 genePRRSV

Identifiers

PMID42655813
PMCPMC13517849

What Socratic holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.