ArticleVeterinary sciences2026
Establishment of RPA-CRISPR/Cas12a Detection Methods for Rapid Largemouth Bass Ranavirus Surveillance.
Haoyu Wang, Yong Zhou, Peng Chen, Liping Zhang, Wen Zhu, Mingyang Xue, Yan Meng, Zhenyu Huang, Chen Xu, Yuding Fan and 2 more
Abstract read
In one paragraphArticle in Veterinary sciences, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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2 · The registryThe trial behind it
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5 · Who and what moneyAuthors and funding
12 authors.
Haoyu WangDivision of Fish Disease, Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan 430223, China.
Yong ZhouDivision of Fish Disease, Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan 430223, China.ORCID 0000-0003-0373-5734 Peng ChenXinjiang Uygur Autonomous Region Fisheries Development Center (Xinjiang Uygur Autonomous Region Fisheries Research Institute), Urumqi 830000, China.
Liping ZhangChongqing Fisheries Technical Extension Center, Chongqing 401120, China.
Wen ZhuDivision of Fish Disease, Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan 430223, China.
Mingyang XueDivision of Fish Disease, Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan 430223, China.ORCID 0000-0003-3370-5712 Yan MengDivision of Fish Disease, Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan 430223, China.ORCID 0000-0002-8846-2191 Zhenyu HuangDivision of Fish Disease, Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan 430223, China.
Chen XuDivision of Fish Disease, Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan 430223, China.ORCID 0000-0002-5075-7154 Yuding FanDivision of Fish Disease, Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan 430223, China.ORCID 0000-0002-7291-2493 Chao PeiEngineering Lab of Henan Province for Aquatic Animal Disease Control, College of Fisheries, Henan Normal University, Xinxiang 453007, China.
Nan JiangDivision of Fish Disease, Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan 430223, China.ORCID 0000-0001-8460-3949 Funding
Central Public-interest Scientific Institution Basal Research Fund 2023TD46National Key Research and Development Program of China 2023YFD2400704Xinjiang Uygur Autonomous Region key research and development task special 2024B02014-2
6 · The paper itselfAbstract
The high mortality associated with largemouth bass ranavirus (LMBRaV) disease requires rapid and sensitive diagnosis methods to prevent virus spread and subsequent outbreak. The recombinase polymerase amplification combined with the CRISPR/Cas12a system (RPA-CRISPR/Cas12a) assay and RPA-CRISPR/Cas12a, coupled with lateral flow dipstick (RPA-CRISPR/Cas12a-LFD) assay for LMBRaV, were established in this study for low viral load surveillance. The major capsid protein (
Indexed as
largemouth bassLMBRaVrapid detectionRPA-CRISPR/Cas12aRPA-CRISPR/Cas12a-LFD
Identifiers
PMID42655845
PMCPMC13517407
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