ArticleChemical & biomedical imaging2026
Label-Free Imaging of Matrix Mineralization in Alginate-Encapsulated Bone Spheroids Using Coherent Raman Scattering Microscopy.
Article in Chemical & biomedical imaging, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
Three-dimensional (3D) cell cultures, such as spheroids, are increasingly used to perform advanced studies on bone matrix mineralization. However, their full characterization remains challenging. Traditional colorimetric and fluorescent assays using dyes, such as Alizarin Red S (ARS) and calcein, are effective in monolayer cell cultures but fail to provide reliable information when used in complex 3D cell constructs. In this study, we investigated the application of Coherent Raman Scattering microscopy for the label-free, comprehensive characterization of extracellular matrix (ECM) mineralization in alginate-encapsulated bone spheroids. After confirming that traditional staining techniques are unreliable for mineral detection in spheroids, Stimulated Raman Scattering (SRS) microscopy was used to detect phosphate-rich mineral deposits at a Raman shift of 960 cm
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