Evidence map›Paper›PMID 42663476›Full record

ArticleApplied and environmental microbiology2026

Semi-automated strain engineering and screening in the thermophilic fungus

Chaohui Gong, Xinyu Yang, Xiucai Zhao, Fufan Gou, Jie Zhou, Wenliang Sun, Tao Sun, Wenlong Liu, Kefen Wang, Xingji Wang and 5 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Applied and environmental microbiology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

15 authors.

Chaohui Gong *College of Biotechnology, Tianjin University of Science and Technology, Tianjin, China.
Xinyu Yang *College of Biotechnology, Tianjin University of Science and Technology, Tianjin, China.
Xiucai ZhaoCollege of Biotechnology, Tianjin University of Science and Technology, Tianjin, China.
Fufan GouState Key Laboratory of Engineering Biology for Low-Carbon Manufacturing, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, China.
Jie ZhouState Key Laboratory of Engineering Biology for Low-Carbon Manufacturing, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, China.
Wenliang SunState Key Laboratory of Engineering Biology for Low-Carbon Manufacturing, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, China.
Tao SunState Key Laboratory of Engineering Biology for Low-Carbon Manufacturing, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, China.
Wenlong LiuCollege of Biotechnology, Tianjin University of Science and Technology, Tianjin, China.
Kefen WangLongda Biotechnology Inc, Linyi, Shandong, China.
Xingji WangLongda Biotechnology Inc, Linyi, Shandong, China.
Yefu ChenCollege of Biotechnology, Tianjin University of Science and Technology, Tianjin, China.
Yihan LiuCollege of Biotechnology, Tianjin University of Science and Technology, Tianjin, China.ORCID 0000-0002-5322-6818
Chaoyou XueState Key Laboratory of Engineering Biology for Low-Carbon Manufacturing, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, China.
Qian LiuState Key Laboratory of Engineering Biology for Low-Carbon Manufacturing, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, China.ORCID 0000-0001-6596-6402
Chaoguang TianState Key Laboratory of Engineering Biology for Low-Carbon Manufacturing, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, China.ORCID 0000-0002-0305-2178

Funding

National Key R&D Program of China 2023YFC3402300National Natural Science Foundation of China U22A20441Strategic Priority Research Program of the Chinese Academy of Sciences XDA0510300Tianjin Major Science and Technology Projects and Engineering Programs 25ZXWCSY00250
6 · The paper itself

Abstract

Thermophilic filamentous fungi are widely used for cellulase production at elevated temperatures; however, efficient recombinant protein production in these organisms remains limited because construction and screening of large transformant populations remain difficult. Here, IMPORTANCE: Filamentous fungi are important industrial hosts, but strain engineering in these organisms is often constrained by low transformation efficiency, labor-intensive colony isolation, and limited screening throughput. In this study, we established a semi-automated engineering workflow in the thermophilic fungus

Indexed as

Fungal ProteinsGenetic EngineeringRecombinant ProteinsSordarialesFungal ProteinsRecombinant Proteinsactivity-based screeningfilamentous fungiMyceliophthora thermophilarecombinant protein productionsemi-automated screeningthermophilic fungal chassis

Identifiers

PMID42663476
PMCPMC13599523

What Socratic holds

Textmetadata
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.