Evidence map›Paper›PMID 42664973›Full record

ArticleNeuron2026

A bifunctional optical reporter for tracking estrogen response dynamics in neurons.

Alexandra L Cara, Eartha Mae Guthman, Aarya Mishra, Alexanne L Hall, Sae Yokoyama, Hannah N Azadi, Ian Gregg, Rosalizbeth M Martinez, Norma P Sandoval, Krisha Aghi and 4 more

Abstract read
PubMed Publisher
In one paragraph

Article in Neuron, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Pregnancy damps thermoregulatory rhythms across timescales through neural estrogen signaling in mice.Proceedings of the National Academy of Sciences of the United States of America · 2026
    Article
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

14 authors.

Alexandra L CaraDepartment of Integrative Biology and Physiology, University of California, Los Angeles, CA 90095, USA.
Eartha Mae GuthmanPrinceton Neuroscience Institute, Princeton University, Princeton, NJ 08544, USA.
Aarya MishraDepartment of Integrative Biology and Physiology, University of California, Los Angeles, CA 90095, USA.
Alexanne L HallDepartment of Psychology, University of British Columbia, Vancouver, BC V6T 1Z4, Canada; Djavad Mowafaghian Centre for Brain Health, University of British Columbia, Vancouver, BC V6T 1Z4, Canada.
Sae YokoyamaPrinceton Neuroscience Institute, Princeton University, Princeton, NJ 08544, USA.
Hannah N AzadiDepartment of Integrative Biology and Physiology, University of California, Los Angeles, CA 90095, USA.
Ian GreggPrinceton Neuroscience Institute, Princeton University, Princeton, NJ 08544, USA.
Rosalizbeth M MartinezDepartment of Integrative Biology and Physiology, University of California, Los Angeles, CA 90095, USA.
Norma P SandovalDepartment of Integrative Biology and Physiology, University of California, Los Angeles, CA 90095, USA.
Krisha AghiDepartment of Integrative Biology and Physiology, University of California, Los Angeles, CA 90095, USA.
Kiran K SomaDepartment of Psychology, University of British Columbia, Vancouver, BC V6T 1Z4, Canada; Djavad Mowafaghian Centre for Brain Health, University of British Columbia, Vancouver, BC V6T 1Z4, Canada; Graduate Program in Neuroscience, University of British Columbia, Vancouver, BC V6T 1Z4, Canada.
Stephanie M CorreaDepartment of Integrative Biology and Physiology, University of California, Los Angeles, CA 90095, USA. Electronic address: stephaniecorrea@ucla.edu.
Annegret L FalknerPrinceton Neuroscience Institute, Princeton University, Princeton, NJ 08544, USA. Electronic address: afalkner@princeton.edu.
J Edward van VeenDepartment of Integrative Biology and Physiology, University of California, Los Angeles, CA 90095, USA. Electronic address: vanveen@ucla.edu.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Methods for tracking the dynamics of estrogen action are limited and not tissue specific; these features make it difficult to directly relate hormonal changes to physiological, behavioral, and neural variables. To address this, we developed the fluorescent bifunctional reporter neuro-seeER ("see estrogen response") that can be used to track changes in estrogen dynamics in vitro and in vivo, including longitudinally in behaving animals. We show that activity requires estrogen receptor expression, is specific to estradiol, and responds dynamically to exogenous estrogen in vivo. Using "snapshot" photometry to track dynamics longitudinally, we detected coordinated exogenous estrogen responses across multiple hypothalamic sites. Finally, we demonstrate that this tool captures endogenous estrogen response dynamics in vivo across the timescale of the estrous cycle, thereby uncovering individual differences in response timing. Together, these findings reveal unexpected cellular and temporal heterogeneity of estrogen responsivity and demonstrate the feasibility of tracking hormonal response alongside neural or behavioral measures.

Indexed as

estrogen receptor alpha (ERα)estrogen signalingfiber photometrygene expressiongenetically encoded reporterhormone dynamicshypothalamusin vivo imagingneuroendocrinologyviral transcriptional reporter

Identifiers

PMID42664973

What Socratic holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.