ArticleIn vivo (Athens, Greece)
Dihydrotestosterone Induces Keratinocyte Apoptosis by Activating ERK Signaling.
Article in In vivo (Athens, Greece). The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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8 authors.
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Abstract
BACKGROUND/
aimDihydrotestosterone (DHT) plays a critical role in hair loss. However, research on DHT has mostly focused on its effects on dermal papilla cells expressing androgen receptor (AR). Accordingly, studies on the roles of DHT in keratinocytes, which undergo active proliferation and cell death during hair cycle progression, are lacking. This study investigated the effects of DHT on extracellular signal-regulated kinase (ERK) and Wnt/β-catenin signaling, which are involved in cell proliferation, cell death, and hair cycle regulation. MATERIALS AND
methodsChanges in human immortalized keratinocyte (HaCaT) cell proliferation were investigated by WST assay after treatment with DHT without fetal bovine serum. Apoptosis induction was investigated using Hoechst33342 staining, immunoblotting, and Annexin V/7AAD staining. The involvement of specific signaling pathways was analyzed using WST assay with AR antagonist, Ras/Raf/mitogen-activated protein kinase kinase (MEK) inhibitor, or glycogen synthase kinase-3β (GSK3β) inhibitors. Changes in mRNA levels were analyzed using quantitative reverse transcription-polymerase chain reaction.
resultsDHT inhibited HaCaT cell proliferation
conclusionDHT induces apoptosis in HaCaT cells through ERK activation, independently of AR, and Wnt/β-catenin pathways. These findings provide a basis for understanding the effects of DHT on hair follicle keratinocytes.
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