ArticleClinical, cosmetic and investigational dermatology2026
Integrated Bulk and Single-Cell Transcriptomics Reveals Cell-Type-Specific Fatty Acid Metabolic Dysregulation and Candidate Biomarkers in Diabetic Foot Ulcers.
Article in Clinical, cosmetic and investigational dermatology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
0 citing papers in PubMed.
No citing paper in PubMed yet.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Background and Objective: Diabetic foot ulcers (DFUs) are debilitating complications of diabetes, leading to severe health outcomes and high healthcare costs. Understanding the molecular mechanisms is crucial for better management. This study aims to explore the roles of fatty acid metabolism (FAM) and the immune landscape in DFUs to identify potential diagnostic biomarkers and therapeutic targets. Methods: Single-cell RNA sequencing (scRNA-seq) data from GSE165816 were analyzed to assess cellular diversity within DFU tissues. GSE7014 and GSE134431 were utilized for differential gene expression and FAM profiling. The study employed fatty acid oxidation (FAO) and synthesis (FAS) evaluations, immune cell infiltration analysis using CIBERSORT, and bioinformatics to delineate FAM-related marker genes. Results: Results indicated elevated FAM levels in DFU compared to healthy skin in scRNA-seq data. Notably, the pronounced increase of FAO in DFU tissues suggested a metabolic adaptation possibly beneficial for energy supply in the harsh ulcer environment. Conversely, FAS was significantly diminished in DFU. Immune profiling unveiled a skewed infiltration pattern with an increased presence of pro-inflammatory M1 macrophages and a decreased anti-inflammatory M2 macrophages, reinforcing the role of inflammation in DFU pathology. Moreover, integration of differential expression analysis with FAM-related genes identified four candidate biomarkers with high transcriptomic discriminatory performance for distinguishing DFU from control samples. These four genes are closely linked to a diverse array of immune cells and functions. Conclusion: The study reveals an association between FAM dysregulation and immune imbalance in DFUs and identifies candidate diagnostic biomarkers and potential therapeutic targets, providing new insights into the metabolic-immune landscape of diabetic foot ulcers.
Indexed as
Identifiers
What Socratic holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.