ArticlePloS one2026
Valorization of orange peel by-products via ultrasound-pretreated enzymatic extraction of soluble dietary fiber: Structural and functional characterization.
Article in PloS one, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
Agro-industrial by-products valorization has attracted increasing attention due to their potential as sustainable sources of bioactive compounds, particularly dietary fibers. In this study, orange peel by-products were used as a raw material for the recovery of soluble dietary fiber via enzymatic extraction and ultrasound-pretreated enzymatic extraction. Ultrasound-pretreated enzymatic extraction achieved a soluble dietary fiber recovery of 26.04 ± 0.81% with approximately 90% purity. The content of phenolic compounds bound to soluble dietary fiber ranged from 3.23 ± 0.03 to 12.15 ± 0.21 mg GAE g-1. Soluble dietary fiber obtained by alkaline extraction exhibited higher water-holding capacity (9.16 ± 0.08 g g -1). Enzymatic extraction caused structural modifications and partial depolymerization, reducing fiber porosity and water affinity. Oil-holding capacity ranged from 1.08 ± 0.12 to 1.24 ± 0.05 g g-1, with no differences among extraction methods. Ultrasound-pretreated enzymatic extraction produced soluble dietary fiber with a more amorphous structure, lower crystallinity, greater surface disruption, and higher thermal stability. Moreover, it is a suitable substrate for the growth of mammalian intestinal symbionts such as Faecalibaculum rodentium and Bacteroides thetaiotaomicron, species recognized as beneficial for host health. Ultrasound pretreatment is a promising strategy for recovering high-value soluble dietary fibers from orange peel by-products, thereby enabling the production of tailored functional ingredients for food applications.
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