Evidence map›Paper›PMID 42681085›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2026

Peptide Nucleic Acid-Fluorescence In Situ Hybridization (PNA-FISH) for the Detection and Spatial Location of Microorganisms.

Ricardo Oliveira, Andreia S Azevedo, Laura Cerqueira, Carina Almeida, Nuno F Azevedo

Abstract read
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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Ricardo OliveiraINIAV - National Institute for Agrarian and Veterinarian Research, Vairão, Vila do Conde, Portugal.
Andreia S AzevedoLEPABE - Laboratory for Process Engineering, Environment, Biotechnology and Energy, ALiCE - Associate Laboratory in Chemical Engineering, Faculty of Engineering, University of Porto, Porto, Portugal.
Laura CerqueiraLEPABE - Laboratory for Process Engineering, Environment, Biotechnology and Energy, ALiCE - Associate Laboratory in Chemical Engineering, Faculty of Engineering, University of Porto, Porto, Portugal.
Carina AlmeidaINIAV - National Institute for Agrarian and Veterinarian Research, Vairão, Vila do Conde, Portugal.
Nuno F AzevedoLEPABE - Laboratory for Process Engineering, Environment, Biotechnology and Energy, ALiCE - Associate Laboratory in Chemical Engineering, Faculty of Engineering, University of Porto, Porto, Portugal. nazevedo@fe.up.pt.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Fluorescence in situ hybridization (FISH) applied to microorganisms is a 30-year-old technology that has evolved to become one of the most established techniques in molecular biology. Traditionally, DNA probes have been used for in situ hybridization. However, peptide nucleic acid (PNA) probes have emerged as a promising alternative, offering better hybridization performance and making FISH procedures simpler and more robust. In this chapter, we describe a universal PNA-FISH protocol for the detection of microorganisms in different types of samples, including enriched suspensions, histological samples, and microbial communities, using microscopy or flow cytometry. This protocol can be easily applied to different microorganisms as a way of identifying in situ relevant microorganisms (including pathogens) and assess their spatial distribution in different types of samples.

Indexed as

BacteriaIn Situ Hybridization, FluorescencePeptide Nucleic AcidsAnimalsPeptide Nucleic AcidsBiofilmsEnriched samplesFISHHistological samplesMicroorganismsPNAPure cultures

Identifiers

PMID42681085

What Socratic holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.