Evidence map›Paper›PMID 42681129›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2026

Determination of the Phloem Mass Flow Velocity and Calculation of the Volumetric Flow Rate.

Alexandra C U Furch, Matthias R Zimmermann, Jonas Nötzold

Abstract read
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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Alexandra C U FurchPlant Physiology, Matthias Schleiden Institute of Genetics, Bioinformatics and Molecular Botany, Faculty of Biological Science, Friedrich-Schiller-University Jena, Jena, Germany. Alexandra.Furch@uni-jena.de.ORCID http://orcid.org/0000-0002-9968-753X
Matthias R ZimmermannPlant Physiology, Matthias Schleiden Institute of Genetics, Bioinformatics and Molecular Botany, Faculty of Biological Science, Friedrich-Schiller-University Jena, Jena, Germany.
Jonas NötzoldPlant Physiology, Matthias Schleiden Institute of Genetics, Bioinformatics and Molecular Botany, Faculty of Biological Science, Friedrich-Schiller-University Jena, Jena, Germany.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Here, we describe a microscopic approach to measure the mass flow velocity and to calculate the volumetric flow rate in the midrib of leaves using a phloem-mobile fluorescent dye. The method requires measurements of the carboxyfluorescein (CF) fluorescence using a fluorescent microscope and the determination of the sieve element (SE) cross-sectional area. The translocated CF within the vascular system is tracked in the leaf using cross-sections and the mass flow velocity is deduced. Additionally, SE cross-sectional areas are measured in the center of the leaf at a high resolution to calculate the volumetric flow rate per SE. This method can be applied to all higher plants. However, as the velocity depends on various parameters like plant species, age, and developmental stage, all data needs to be collected and analyzed in each experiment.

Indexed as

PhloemBiological TransportFluoresceinsFluorescent DyesMicroscopy, FluorescencePlant Leaves6-carboxyfluoresceinFluoresceinsFluorescent DyesCarboxyfluoresceinFluorescence microscopeSieve elementsTransport velocityVolumetric flow rate

Identifiers

PMID42681129

What Socratic holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.