Evidence map›Paper›PMID 42681187›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2026

Generation of Diversely Functionalized DNA Libraries Using Ligase-Catalyzed Oligonucleotide Polymerization (LOOPER).

Natalie Khamissi, Luca Rustico, Ryan Hili

Abstract read
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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Natalie KhamissiDepartment of Chemistry, Centre for Research on Biomolecular Interactions, York University, Toronto, ON, Canada.
Luca RusticoDepartment of Chemistry, Centre for Research on Biomolecular Interactions, York University, Toronto, ON, Canada.
Ryan HiliDepartment of Chemistry, Centre for Research on Biomolecular Interactions, York University, Toronto, ON, Canada. rhili@yorku.ca.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Ligase-catalyzed oligonucleotide polymerization (LOOPER) enables the synthesis of highly modified nucleic acid libraries, which can be used as pools to evolve functional nucleic acids, such as aptamers. Here, we describe the process to generate a LOOPER-derived ssDNA library comprising 16 different chemical modifications installed in a sequence-defined manner. The preparation of the building blocks, their templated polymerization, and strand separation to generate the modified ssDNA library are outlined.

Indexed as

DNA LigasesDNA, Single-StrandedGene LibraryLigasesOligonucleotidesAptamers, NucleotidePolymerizationAptamers, NucleotideDNA LigasesDNA, Single-StrandedLigasesOligonucleotidesAptamersCombinatorial libraryDNA templatedLigationModified nucleic acids

Identifiers

PMID42681187

What Socratic holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.