Evidence map›Paper›PMID 42681393›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2026

Purification and Characterization of Extracellular Vesicles from Embryo-Conditioned Medium.

Krishna Chaitanya Pavani, Ann Van Soom, Katrien Smits

Abstract read
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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Krishna Chaitanya PavaniDepartment of Internal Medicine, Reproduction and Population medicine, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium.
Ann Van SoomDepartment of Internal Medicine, Reproduction and Population medicine, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium.
Katrien SmitsDepartment of Internal Medicine, Reproduction and Population medicine, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium. Katrien.Smits@UGent.be.ORCID http://orcid.org/0000-0002-8205-3725

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Extracellular vesicles (EVs) serve as key communication signals among embryos. They are secreted into the embryo culture medium and hold potential as biomarkers for assessing embryo quality. However, the limited volume of embryo-conditioned medium presents challenges for EV enrichment. This chapter details an effective method for purifying and characterizing EVs from in vitro embryo cultures by using the OptiPrep™ density gradient ultracentrifugation (ODG) method. Additionally, we also provide methods for preparing EV-free embryo culture media.

Indexed as

Embryo, MammalianExtracellular VesiclesAnimalsCentrifugation, Density GradientCulture Media, ConditionedEmbryo Culture TechniquesHumansCulture Media, ConditionedEmbryo culture mediaEV characterizationEV-free mediaEV purificationExtracellular vesiclesOptiPrep™ Density gradient

Identifiers

PMID42681393

What Socratic holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.