Evidence map›Paper›PMID 42681421›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2026

Determining the Binding Affinity in Monoclonal Antibody-Alloantigen Interactions.

Kashyap R Patel, Ryan P Jajosky, Victoria M Vance, Caitlin L Cepeda, Vivien C Lee, Sean R Stowell, Connie M Arthur

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Article in Methods in molecular biology (Clifton, N.J.), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0cells of the map it votes in
0citing papers in PubMed
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Kashyap R PatelDivision of Transfusion Medicine, Mass General Brigham, Harvard Medical School, Boston, MA, USA.
Ryan P JajoskyDivision of Transfusion Medicine, Mass General Brigham, Harvard Medical School, Boston, MA, USA.
Victoria M VanceDivision of Transfusion Medicine, Mass General Brigham, Harvard Medical School, Boston, MA, USA.
Caitlin L CepedaDivision of Transfusion Medicine, Mass General Brigham, Harvard Medical School, Boston, MA, USA.
Vivien C LeeDivision of Transfusion Medicine, Mass General Brigham, Harvard Medical School, Boston, MA, USA.
Sean R StowellDivision of Transfusion Medicine, Mass General Brigham, Harvard Medical School, Boston, MA, USA.
Connie M ArthurDivision of Transfusion Medicine, Mass General Brigham, Harvard Medical School, Boston, MA, USA. cmarthur@bwh.harvard.edu.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Humoral immunity, a significant facet of the adaptive immune system, neutralizes targets when circulating antigen-specific antibodies recognize a foreign antigen and recruit antibody-dependent effector systems. Antibody-mediated complement deposition and cellular removal through engagement of Fc receptors can mediate intravascular and extravascular hemolysis following an incompatible red blood cell (RBC) transfusion. Efficiency of hemolysis is influenced by the affinity of the antibody-antigen reaction. Therefore, determining the affinities of alloantibodies can be important when investigating the potential consequences of antibody engagement on RBC clearance and possible changes to the target antigen. Here, we describe three techniques, surface plasmon resonance, protein microarray, and flow cytometry, to distinguish affinities of monoclonal antibodies specific to the Hen Egg Lysozyme, Ovalbumin, and Duffy (HOD) fusion model antigen.

Indexed as

Antibodies, MonoclonalAntibody AffinityIsoantigensAnimalsFlow CytometryHumansMuramidaseOvalbuminProtein Array AnalysisProtein BindingSurface Plasmon ResonanceAntibodies, Monoclonalhen egg lysozymeIsoantigensMuramidaseOvalbuminBinding affinityFlow cytometryProtein microarraySurface plasmon resonance

Identifiers

PMID42681421

What Socratic holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.