Evidence map›Paper›PMID 42691043›Full record

ArticlePloS one2026

Synaptopodin KO rat for assessing the dendritic spine apparatus and axonal cisternal organelle in synaptic plasticity, development, and behavior.

Masaaki Kuwajima, Olga I Ostrovskaya, Lyndsey M Kirk, Ashley Alario, Weiling Yin, Sonia Singh, Anna Xaymongkhol, Adrienne Li, Ella Prasad, Kristen M Harris

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in PloS one, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

  • Update of
    2025
5 · Who and what money

Authors and funding

10 authors.

Masaaki KuwajimaDepartment of Neuroscience and Center for Learning and Memory, The University of Texas at Austin, Austin, Texas, United States of America.ORCID https://orcid.org/0000-0002-1478-3726
Olga I OstrovskayaDepartment of Neuroscience and Center for Learning and Memory, The University of Texas at Austin, Austin, Texas, United States of America.ORCID https://orcid.org/0000-0002-8565-6273
Lyndsey M KirkDepartment of Neuroscience and Center for Learning and Memory, The University of Texas at Austin, Austin, Texas, United States of America.ORCID https://orcid.org/0000-0002-5125-652X
Ashley AlarioDepartment of Neuroscience and Center for Learning and Memory, The University of Texas at Austin, Austin, Texas, United States of America.ORCID https://orcid.org/0009-0005-1151-2954
Weiling YinDepartment of Neuroscience and Center for Learning and Memory, The University of Texas at Austin, Austin, Texas, United States of America.ORCID https://orcid.org/0009-0006-5574-587X
Sonia SinghDepartment of Neuroscience and Center for Learning and Memory, The University of Texas at Austin, Austin, Texas, United States of America.ORCID https://orcid.org/0009-0003-6490-8907
Anna XaymongkholDepartment of Neuroscience and Center for Learning and Memory, The University of Texas at Austin, Austin, Texas, United States of America.
Adrienne LiDepartment of Neuroscience and Center for Learning and Memory, The University of Texas at Austin, Austin, Texas, United States of America.ORCID https://orcid.org/0009-0001-1510-3294
Ella PrasadDepartment of Neuroscience and Center for Learning and Memory, The University of Texas at Austin, Austin, Texas, United States of America.
Kristen M HarrisDepartment of Neuroscience and Center for Learning and Memory, The University of Texas at Austin, Austin, Texas, United States of America.ORCID https://orcid.org/0000-0002-1943-4744

Funding

Synaptic plasticity across the lifespanR01MH095980 · NIMH · UNIVERSITY OF TEXAS AT AUSTIN · PI KRISTEN M HARRIS · 2012 to 2026
$5.0M
Spine and Synaptic Plasticity in Mature HippocampusR37NS021184 · NINDS · UNIVERSITY OF TEXAS AT AUSTIN · PI HARRIS, KRISTEN M · 2005 to 2011
$2.2M
Synapse growth and elimination in mature CNSR01MH104319 · NIMH · UNIVERSITY OF TEXAS AT AUSTIN · PI HARRIS, KRISTEN M · 2014 to 2018
$1.8M
ONTOGENY OF STRUCTURE &FUNCTION AT HIPPOCAMPAL SYNAPSESR01NS033574 · NINDS · UNIVERSITY OF TEXAS AUSTIN · PI HARRIS, KRISTEN M · 1996 to 2005
$1.5M
SPINES SYNAPSES AND THE ONTOGENY OF A MEMORY MECHANISMR01NS021184 · NINDS · MEDICAL COLLEGE OF GEORGIA (MCG) · PI HARRIS, KRISTEN M · 1988 to 2003
$1.4M
NIMH NIH HHS R01 MH095980NIMH NIH HHS R01 MH104319NINDS NIH HHS R01 NS021184NINDS NIH HHS R01 NS033574NINDS NIH HHS R37 NS021184
6 · The paper itself

Abstract

The actin-binding protein synaptopodin (Synpo) regulates the cytoskeleton and organization of the endoplasmic reticulum, thereby amplifying intracellular Ca2+ signaling. Knockout (KO) mouse models have been used to study the role of Synpo in kidney and brain functions, where it supports stress fiber formation, as well as long-term potentiation (LTP) and learning, respectively. Here, we generated Synpo KO rats using CRISPR-Cas9, and show they are viable but have reduced body weight after postnatal days 35-45, along with shorter limb bone length. Their basal kidney function is normal into early adulthood. Serial section electron microscopy from Synpo KO rat hippocampus reveals the absence of the spine apparatus in dendrites and cisternal organelle in the axon initial segment (AIS), two Synpo-dependent specializations of smooth endoplasmic reticulum. The AIS in KO was still innervated by inhibitory synapses despite the total loss of the cisternal organelle. Synpo KO rats also showed reduced LTP. Previously unknown KO effect of Synpo on body stature could have an inadvertent impact on behavioral outcomes. Furthermore, rats have a well-defined developmental onset of LTP, compared to the variable onset of LTP in mice. This, combined with known species differences in behavior, makes our KO rat model a valuable resource for assessing the role of Synpo in development, learning, synaptic plasticity, and a wide range of other biological functions.

Indexed as

AxonsBehavior, AnimalDendritic SpinesMicrofilament ProteinsNeuronal PlasticityAnimalsFemaleGene Knockout TechniquesHippocampusLong-Term PotentiationMaleRatsSynapsesMicrofilament ProteinsSynpo protein, rat

Identifiers

PMID42691043
PMCPMC13541181

What Socratic holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.