Development of an RT-LAMP-CRISPR/Cas12a-based nucleic acid detection platform for rapid diagnosis of porcine reproductive and respiratory syndrome virus type 2.

Congcong Li, Changsheng Sun, Qiuliang Xu

Abstract read

In one paragraph

Article in Veterinary and animal science, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it

0cells of the map it votes in

0citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Congcong LiCollege of Animal Science and Technology, Henan University of Animal Husbandry and Economy, Zhengzhou, Henan, China.

Changsheng SunCollege of Animal Science and Technology, Henan University of Animal Husbandry and Economy, Zhengzhou, Henan, China.

Qiuliang XuCollege of Animal Science and Technology, Henan University of Animal Husbandry and Economy, Zhengzhou, Henan, China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Porcine reproductive and respiratory syndrome virus (PRRSV) significantly affects swine production, leading to reproductive failure and respiratory disorders. Current detection methods are limited by slow turnaround times, insufficient sensitivity and specificity, the need for skilled personnel, and costly equipment, which hinder their application in rapid on-site diagnosis and early disease prevention and control. In this study, a novel detection platform was developed by integrating the target specificity of RT-LAMP primers with the dual recognition capability of CRISPR/Cas12a. Optimal crRNA primers targeting conserved sequences in the PRRSV-2 structural protein M gene were designed and screened. Reaction conditions were subsequently optimized, and a cloning vector containing the target gene was constructed. Sensitivity evaluation using PCR, LAMP-CRISPR/Cas12a, and qPCR revealed detection limits of 9.17 × 10

Indexed as

CRISPR/Cas12aNucleic acid detectionPRRSV-2RT-LAMPVisualization

Identifiers

PMID42707136

DOI10.1016/j.vas.2026.100836

PMCPMC13546912

What Socratic holds

Textmetadata

Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.