ArticleSTAR protocols2026
Protocol for assessing endolysosomal chloride channel function in mammalian cells using endolysosomal patch-clamp technique.
Article in STAR protocols, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
Here, we present a protocol for assessing endolysosomal chloride channel function in mammalian cells using the endolysosomal patch-clamp technique. We describe steps for vesicle enlargement, fabrication and fire-polishing of high-resistance pipettes, and mechanical dissection and isolation of enlarged vesicles. We then detail procedures for giga-seal formation and configuration to whole-endolysosome modes. For complete details on the use and execution of this protocol, please refer to Wang et al.
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