Evidence map›Paper›PMID 42712977›Full record

ArticleiMeta2026

Decoding sequence recognition code of nucleic acid-binding proteins of human-infecting DNA viruses.

Meifang Tang, Shifei Yang, Haiqian Ma, Ruoying Guo, Jie Chen, Hui Zhang, Jinghan Zhang, Wei Sun, Yun Wei, Ligang Fan and 1 more

Abstract readLetter
In one paragraph

Article in iMeta, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Meifang TangMinistry of Education Key Laboratory of Resource Biology and Biotechnology in Western China; Shaanxi Provincial Key Laboratory of Biotechnology; School of Medicine Northwest University Xi'an China.ORCID https://orcid.org/0009-0002-9780-7232
Shifei YangMinistry of Education Key Laboratory of Resource Biology and Biotechnology in Western China; Shaanxi Provincial Key Laboratory of Biotechnology; School of Medicine Northwest University Xi'an China.ORCID https://orcid.org/0009-0007-3732-4024
Haiqian MaMinistry of Education Key Laboratory of Resource Biology and Biotechnology in Western China; Shaanxi Provincial Key Laboratory of Biotechnology; School of Medicine Northwest University Xi'an China.ORCID https://orcid.org/0009-0002-5305-6975
Ruoying GuoMinistry of Education Key Laboratory of Resource Biology and Biotechnology in Western China; Shaanxi Provincial Key Laboratory of Biotechnology; School of Medicine Northwest University Xi'an China.ORCID https://orcid.org/0009-0007-2784-5440
Jie ChenTung Biomedical Sciences Centre, Department of Biomedical Sciences, College of Biomedicine City University of Hong Kong Hong Kong China.ORCID https://orcid.org/0009-0008-5301-1092
Hui ZhangMinistry of Education Key Laboratory of Resource Biology and Biotechnology in Western China; Shaanxi Provincial Key Laboratory of Biotechnology; School of Medicine Northwest University Xi'an China.ORCID https://orcid.org/0009-0004-9375-2099
Jinghan ZhangMinistry of Education Key Laboratory of Resource Biology and Biotechnology in Western China; Shaanxi Provincial Key Laboratory of Biotechnology; School of Medicine Northwest University Xi'an China.ORCID https://orcid.org/0009-0007-0948-600X
Wei SunMinistry of Education Key Laboratory of Resource Biology and Biotechnology in Western China; Shaanxi Provincial Key Laboratory of Biotechnology; School of Medicine Northwest University Xi'an China.ORCID https://orcid.org/0009-0009-9885-0890
Yun WeiMinistry of Education Key Laboratory of Resource Biology and Biotechnology in Western China; Shaanxi Provincial Key Laboratory of Biotechnology; School of Medicine Northwest University Xi'an China.ORCID https://orcid.org/0009-0002-2111-6590
Ligang FanMinistry of Education Key Laboratory of Resource Biology and Biotechnology in Western China; Shaanxi Provincial Key Laboratory of Biotechnology; School of Medicine Northwest University Xi'an China.ORCID https://orcid.org/0000-0003-4889-9767
Jian YanMinistry of Education Key Laboratory of Resource Biology and Biotechnology in Western China; Shaanxi Provincial Key Laboratory of Biotechnology; School of Medicine Northwest University Xi'an China.ORCID https://orcid.org/0000-0002-1267-2870

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Human-infecting DNA viruses remain major health threats, yet the DNA-recognition mechanisms of their nucleic acid-binding proteins (NBPs) are poorly understood. Here, we systematically profiled 103 viral NBPs from human-infecting DNA viruses, with three NBPs from non-human-infecting DNA viruses as controls, using high-throughput screening. This analysis identified diverse DNA-binding motifs and specificity modules, including convergent recognition of a conserved CCACC motif across phylogenetically distant viruses. Notably, viral NBP binding-site distributions varied with genome size, and several NBPs from small-genome viruses showed enrichment on mitochondrial DNA. Functional assays further supported their mitochondrial association and effects on mitochondrial membrane potential. By integrating an ivTRT-based ssDNA-SELEX workflow, we further found that ssDNA viral NBPs recognize dimer-like and inverted-repeat sequences with potential to form stem-loop structures. Collectively, this study constructs a comprehensive viral NBP DNA-recognition atlas, offering a fundamental resource for elucidating viral genome recognition mechanisms, virus-mitochondria interactions, and developing future antiviral strategies.

Identifiers

PMID42712977
PMCPMC13550974

What Socratic holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.