Evidence map›Paper›PMID 42736199›Full record

ArticlePoultry science2026

Dietary supplementation of garlic leaf extract enhances growth performance and intestinal health, immunity, and gut microbiota in broiler chickens.

Sarana Rose Sommano, Piyachat Sunanta, Chiao-Hsu Ke, Yu-Lei Wang, Raktham Mektrirat, Aye Chan Moe, Achmad Muzakky Dityana, Chompunut Lumsangkul

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Poultry science, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Sarana Rose SommanoBioactive Compound Laboratory, Faculty of Agriculture, Chiang Mai University, Chiang Mai, 50200, Thailand.
Piyachat SunantaBioactive Compound Laboratory, Faculty of Agriculture, Chiang Mai University, Chiang Mai, 50200, Thailand; Multidisciplinary Research Institute, Chiang Mai University, Chiang Mai, 50200, Thailand.
Chiao-Hsu KeDepartment of Veterinary Medicine, School of Veterinary Medicine, National Taiwan University, Taipei City 106, Taiwan.
Yu-Lei WangVeterinary Academic Office, Faculty of Veterinary Medicine, Chiang Mai University, Chiang Mai, 50100, Thailand.
Raktham MektriratVeterinary Academic Office, Faculty of Veterinary Medicine, Chiang Mai University, Chiang Mai, 50100, Thailand; Chinese-Thai Collaborative Laboratory for Veterinary Acupuncture and Herbal Pharmacotherapy, Faculty of Veterinary Medicine, Chiang Mai University, Chiang Mai, 50100, Thailand; Center of Excellence in Pharmaceutical Nanotechnology, Faculty of Pharmacy, Chiang Mai University, Chiang Mai, 50100, Thailand.
Aye Chan MoeMonogastric Immunonutrition Laboratory, Department of Animal Science, National Chung Hsing University, Taichung, 402, Taiwan; International Master Program of Agriculture, National Chung Hsing University, Taichung, 402, Taiwan.
Achmad Muzakky DityanaMonogastric Immunonutrition Laboratory, Department of Animal Science, National Chung Hsing University, Taichung, 402, Taiwan; International Doctoral Program in Agriculture, College of Agriculture and Natural Resources, National Chung Hsing University, Taichung, Taiwan.
Chompunut LumsangkulMonogastric Immunonutrition Laboratory, Department of Animal Science, National Chung Hsing University, Taichung, 402, Taiwan; Department of Animal Science, National Chung Hsing University, Taichung, 402, Taiwan; The iEGG and Animal Biotechnology Research Center, National Chung Hsing University, Taichung, 402, Taiwan. Electronic address: lumsangkul@nchu.edu.tw.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The poultry industry has increasingly sought natural feed additives as alternatives to synthetic growth promoters to enhance productivity and animal health in a sustainable manner. Garlic and its bioactive compounds have been widely recognised for their antimicrobial, antioxidant, and immunomodulatory properties, yet limited information is available regarding the efficacy of garlic leaf extract (GLE) in broiler diet. This study evaluated the effects of GLE as a natural dietary additive on growth performance, intestinal morphology, immune response, and gut microbiota composition in broiler chickens. A total of 160 one-day-old broilers were randomly assigned to 4 dietary treatments supplemented with 0, 125, 250, or 500 mg/kg of GLE for 35 days. Results revealed that dietary inclusion of 125 mg/kg GLE significantly increased final body weight and average daily gain compared with the control group. Supplementation with GLE also improved villus height in the jejunum and ileum suggesting enhanced nutrient absorption and intestinal integrity. The GLE treatment group exhibited significantly greater than that observed in the other experimental groups. In contrast, the concentrations of IgM and IgY did not differ significantly among treatments. Analysis of 16S rRNA gene sequences indicated that dietary GLE significantly altered the composition and structure of the intestinal microbiota, characterised by an increased relative abundance of Firmicutes, a reduction in Bacteroidetes, and enrichment of beneficial genera such as Lactobacillus. Principal coordinate and LEfSe analyses confirmed these compositional shifts. In conclusion, dietary inclusion of garlic leaf extract, particularly at 125 mg/kg, effectively enhanced growth performance, intestinal morphology, immunity, and gut microbial balance, supporting its potential as a natural feed additive in broiler production.

Indexed as

Broiler chickenGarlic leaf extractGut healthGut microbiotaImmune response

Identifiers

PMID42736199
PMCPMC13594868

What Socratic holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.