Evidence map›Paper›PMID 42749865›Full record

ArticleMetabolomics : Official journal of the Metabolomic Society2026

Comparative analysis of amino acid profiles across pediatric groups stratified by asthma and IgE-dependent allergy showed differences in serum asparagine levels.

Natalia Rzetecka-Mańka, Martyna Pajewska-Szmyt, Joanna Matysiak, Paulina Sobkowiak, Irena Wojsyk-Banaszak, Anna Bręborowicz, Szymon Plewa, Dagmara Pietkiewicz, Jan Matysiak, Agnieszka Klupczyńska-Gabryszak

Abstract readComparative Study
In one paragraph

Article in Metabolomics : Official journal of the Metabolomic Society, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0citing papers in PubMed
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1 · What the graph read from it

What it found

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Natalia Rzetecka-MańkaDepartment of Inorganic and Analytical Chemistry, Poznan University of Medical Sciences, Poznan, Poland.ORCID http://orcid.org/0000-0001-9880-0740
Martyna Pajewska-SzmytDepartment of Inorganic and Analytical Chemistry, Poznan University of Medical Sciences, Poznan, Poland.ORCID http://orcid.org/0000-0003-2308-2750
Joanna MatysiakFaculty of Medicine and Health Sciences, University of Kalisz, Kalisz, Poland.ORCID http://orcid.org/0000-0002-2475-1066
Paulina SobkowiakDepartment of Pulmonology, Pediatric Allergy and Clinical Immunology, Poznan University of Medical Sciences, Poznan, Poland.ORCID http://orcid.org/0000-0003-2737-8946
Irena Wojsyk-BanaszakDepartment of Pulmonology, Pediatric Allergy and Clinical Immunology, Poznan University of Medical Sciences, Poznan, Poland.ORCID http://orcid.org/0000-0001-6724-1499
Anna BręborowiczDepartment of Pulmonology, Pediatric Allergy and Clinical Immunology, Poznan University of Medical Sciences, Poznan, Poland.ORCID http://orcid.org/0000-0001-7811-7565
Szymon PlewaDepartment of Inorganic and Analytical Chemistry, Poznan University of Medical Sciences, Poznan, Poland.ORCID http://orcid.org/0000-0002-9600-3980
Dagmara PietkiewiczDepartment of Inorganic and Analytical Chemistry, Poznan University of Medical Sciences, Poznan, Poland.ORCID http://orcid.org/0000-0001-8163-6825
Jan MatysiakDepartment of Inorganic and Analytical Chemistry, Poznan University of Medical Sciences, Poznan, Poland.ORCID http://orcid.org/0000-0002-9993-1504
Agnieszka Klupczyńska-GabryszakDepartment of Inorganic and Analytical Chemistry, Poznan University of Medical Sciences, Poznan, Poland. aklupczynska@ump.edu.pl.ORCID http://orcid.org/0000-0002-5028-1408

Funding

Narodowe Centrum Nauki 2021/43/O/NZ5/00480
6 · The paper itself

Abstract

backgroundAlthough childhood asthma and IgE-dependent allergy frequently co-occur, the characterization of amino acid profiles associated with these conditions remains incomplete. The diagnosis of asthma and IgE-dependent allergy in children under five years of age is mainly based on clinical criteria, repeated specialist assessments, therapeutic response, and the exclusion of alternative causes, with functional testing performed when feasible. Therefore, identifying molecular indicators could improve the characterization and diagnosis of asthma in pediatric patients. MATERIALS AND

methodsThe study included 113 serum samples from pediatric children. The study examined a comprehensive profile of free amino acids among four groups of children aged six months to five years. Three study groups were analyzed: children with asthma, children with asthma and IgE-dependent allergies, and children with IgE-dependent allergies along with one control group without asthma and IgE-dependent allergy. The targeted metabolomic analysis was performed using the Chromsystems kit. Samples were analyzed using a triple quadrupole tandem mass spectrometer coupled with a high-performance liquid chromatograph. Then, univariate statistical analyses were performed.

resultsThe method enabled quantification of 26 amino acids. The statistical analysis was conducted using the Mann-Whitney U and t-test. A significant difference in one amino acid concentration was found between patients with asthma and those with asthma and IgE-dependent allergy - asparagine (p-value 0.025). In addition, two amino acids distinguished the children with asthma from the control group: alpha-aminobutyric acid (p-value 0.011) and asparagine (p-value 0.017).

conclusionsThe research suggests a potential connection between asparagine levels and childhood asthma, as well as asthma with IgE-dependent allergy. This study involving four groups of pediatric patients may provide a basis for further scientific research into the role of amino acids, particularly asparagine, in n the molecular mechanisms underlying asthma.

Indexed as

Amino AcidsAsparagineAsthmaHypersensitivityImmunoglobulin EChild, PreschoolChromatography, High Pressure LiquidFemaleHumansInfantMaleMetabolomicsTandem Mass SpectrometryAmino AcidsAsparagineImmunoglobulin EAllergyAlpha-aminobutyric acidAmino acidsAsparagineAsthmaPediatric

Identifiers

PMID42749865
PMCPMC13582370

What Socratic holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.