Acute TGFα drives OA-associated transcriptional and metabolic remodelling in primary chondrocytes.

Emily L White, Emily A Day, C Thomas Appleton, Matthew W Grol, Frank Beier

Abstract read

In one paragraph

Article in Osteoarthritis and cartilage open, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it

0cells of the map it votes in

0citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Emily L WhiteDepartment of Physiology and Pharmacology, Schulich School of Medicine & Dentistry, University of Western Ontario, London, ON, Canada.

Emily A DayDepartment of Physiology and Pharmacology, Schulich School of Medicine & Dentistry, University of Western Ontario, London, ON, Canada.

C Thomas AppletonDepartment of Physiology and Pharmacology, Schulich School of Medicine & Dentistry, University of Western Ontario, London, ON, Canada.

Matthew W GrolDepartment of Physiology and Pharmacology, Schulich School of Medicine & Dentistry, University of Western Ontario, London, ON, Canada.

Frank BeierDepartment of Physiology and Pharmacology, Schulich School of Medicine & Dentistry, University of Western Ontario, London, ON, Canada.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Objectives: Articular cartilage degeneration is a hallmark of osteoarthritis (OA); however, underlying molecular mechanisms remain poorly understood. Transforming growth factor alpha (TGFα) and chondrocyte metabolism have been independently implicated in OA pathogenesis; in this study, we investigated whether TGFα affects chondrocyte metabolism. Design: Immature murine articular chondrocytes (iMACs) were isolated from 4-6-day-old CD1 mice and treated with TGFα or vehicle control (ddH2O) for 24-72 h. Global transcriptional changes were assessed at 48 h by bulk RNA sequencing ( Results: TGFα treatment led to differential expression of 1643 genes (1305 upregulated, 338 downregulated), with enrichment in processes related to Conclusion: Acute TGFα exposure induces transcriptional changes associated with ROS, glycolysis, and inflammatory signalling. TGFα decreases mitochondrial membrane potential, while increasing glycolysis in the presence of OA synovial fluid, supporting a link between TGFα signalling and metabolic reprogramming.

Indexed as

Cell metabolismChondrocyteEGFRMitochondriaOsteoarthritisTGFα

Identifiers

PMID42756972

DOI10.1016/j.ocarto.2026.100873

PMCPMC13583744

What Socratic holds

Textmetadata

Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.