Evidence map›Paper›PMID 42770299›Full record

ArticleJCI insight2026

Neutrophils promote endothelial cell activation in pediatric Mycoplasma pneumoniae pneumonia.

Xia Huang, Yifan Zhu, Yun Guo, Tian Lv, Haiyan Gu, Yingying Luo, Dan Li, Hang Sun, Deyu Zhao, Feng Liu

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in JCI insight, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Xia HuangDepartment of Respiratory Medicine, Children's Hospital of Nanjing Medical University, Nanjing, China.
Yifan ZhuDepartment of Respiratory Medicine, Children's Hospital of Nanjing Medical University, Nanjing, China.
Yun GuoDepartment of Respiratory Medicine & Clinical Allergy Center, Affiliated Children's Hospital of Jiangnan University (Wuxi Children's Hospital), Wuxi, China.
Tian LvDepartment of Respiratory Medicine, Children's Hospital of Nanjing Medical University, Nanjing, China.
Haiyan GuDepartment of Respiratory Medicine, Children's Hospital of Nanjing Medical University, Nanjing, China.
Yingying LuoDepartment of Respiratory Medicine, Children's Hospital of Nanjing Medical University, Nanjing, China.
Dan LiDepartment of Respiratory Medicine, Children's Hospital of Nanjing Medical University, Nanjing, China.
Hang SunDepartment of Respiratory Medicine, Children's Hospital of Nanjing Medical University, Nanjing, China.
Deyu ZhaoDepartment of Respiratory Medicine, Children's Hospital of Nanjing Medical University, Nanjing, China.
Feng LiuDepartment of Respiratory Medicine, Children's Hospital of Nanjing Medical University, Nanjing, China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Mycoplasma pneumoniae pneumonia (MPP) can cause serious extrapulmonary complications, including life-threatening thrombosis. This study aimed to elucidate the roles of neutrophils and neutrophil extracellular traps (NETs) in vascular endothelial cell (EC) activation in pediatric MPP-associated thrombosis. We analyzed the relationship between neutrophils and thrombosis in children with MPP and used mouse models of neutrophilia (Csf3 plasmid injection), neutropenia (Csf3 deficient, Csf3-/-), and defective NETs formation (Pad4 deficient, Pad4-/-). The effects of neutrophils and NETs on EC activation were further examined in vivo, in vitro, and in human samples. Elevated neutrophil count was observed in patients with thrombosis and functioned as a potential diagnostic marker as well as a risk factor for MPP-associated thrombosis. EC activation was enhanced in MPP mice with neutrophilia but attenuated in neutropenic or Pad4-/- mice. NETs activated ECs through TLR2 and JAK/STAT3 signaling, and inhibition of NETs formation (Cl-amidine), TLR2 (C29), and JAK (upadacitinib) each attenuated this response. Strong correlations among neutrophils, NETs, EC activation, and thrombosis were observed in pediatric patients. These findings suggest that neutrophils promoted thrombosis in MPP via NETs-mediated EC activation involving TLR2 and JAK/STAT3 signaling. This study provides mechanistic insights into the inflammatory-thrombotic processes in MPP-associated thrombosis and offers a rationale for further investigation of neutrophils, NETs, TLR2, and JAK/STAT3 signaling in this context.

Indexed as

Endothelial CellsNeutrophilsPneumonia, MycoplasmaThrombosisAnimalsChildChild, PreschoolDisease Models, AnimalExtracellular TrapsFemaleHumansMaleMiceMice, KnockoutMycoplasma pneumoniaeSignal TransductionEndothelial cellsImmunologyInfectious diseaseNeutrophilsThrombosis

Identifiers

PMID42770299
PMCPMC13596722

What Socratic holds

Textmetadata
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.