Evidence map›Paper›PMID 42773442›Full record

ArticleBMC veterinary research2026

Improving the cryo-resistance of Egyptian buffalo semen via chlorogenic acid and rutin supplementation: molecular docking, antioxidant, and anti-apoptotic pathways.

Wael A Khalil, Aya A Ismail, Mohamed M Hegazy, Mostafa A El-Harairy, Amany Assem Elkashef, Mahmoud Moussa, Sameh A Abdelnour

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Article in BMC veterinary research, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Wael A KhalilDepartment of Animal Production, Faculty of Agriculture, Mansoura University, Mansoura, 35516, Egypt. w-khalil@mans.edu.eg.
Aya A IsmailDepartment of Animal Production, Faculty of Agriculture, Mansoura University, Mansoura, 35516, Egypt.
Mohamed M HegazyAnimal Production Research Institute, Agriculture Research Centre, Ministry of Agriculture, Dokki, Giza, 12619, Egypt.
Mostafa A El-HarairyDepartment of Animal Production, Faculty of Agriculture, Mansoura University, Mansoura, 35516, Egypt.
Amany Assem ElkashefChemistry Department, Faculty of Science, Alexandria University, Alexandria, Egypt.
Mahmoud MoussaEmirates Smart Camel Center, Umm Al Quwain, United Arab Emirates.
Sameh A AbdelnourDepartment of Animal Production, Faculty of Agriculture, Zagazig University, Zagazig, 44511, Egypt. samehtimor86@gmail.com.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

This study compared the antioxidant effects of chlorogenic acid (CGA) and rutin (RU) supplementation in freezing extenders on the cryo-resistance of buffalo spermatozoa. Over eight weeks, 56 ejaculates were collected from seven fertile buffalo (Bubalus bubalis) bulls. To eliminate individual animal variability, high-quality ejaculates were pooled and extended in a Tris-egg yolk medium. The pooled semen was divided into three treatment groups: control (no additive), 0.6 mM RU, and 150 µM CGA. All groups were cryopreserved using a standard protocol and stored in liquid nitrogen for one month prior to post-thaw analysis. The findings revealed that the addition of either RU or CGA substantially enhanced post-thaw sperm viability, progressive motility, plasma membrane integrity, and acrosomal integrity compared with the control group (P < 0.05). Furthermore, both treatments improved sperm kinematics and antioxidant profiles by significantly increasing total antioxidant capacity and catalase levels (P < 0.05). This was accompanied by a significant decline in oxidative markers (MDA, ROS, and H

Indexed as

AntioxidantsBuffaloesChlorogenic AcidCryopreservationRutinSemen PreservationAnimalsApoptosisCryoprotective AgentsMaleMolecular Docking SimulationSemenSpermatozoaSperm MotilityAntioxidantsChlorogenic AcidCryoprotective AgentsRutinAntioxidantApoptosisBuffalo semenCryo-damagesMicrobial loadMolecular docking

Identifiers

PMID42773442
PMCPMC13595626

What Socratic holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.