Evidence map›Paper›PMID 42780876›Full record

ArticleTalanta open2026

An amplification-free fluorescence turn-on sensing platform for in situ monitoring miRNA release from live cells.

Avinash Kumar, Jing Qu, Oluwatoyin V Odubanjo, Felicite K Noubissi, Yi-Ming Liu

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Talanta open, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Avinash KumarDepartment of Chemistry, Physics, and Atmospheric Science, Jackson State University, Jackson, MS 39217, USA.
Jing QuDepartment of Chemistry, Physics, and Atmospheric Science, Jackson State University, Jackson, MS 39217, USA.
Oluwatoyin V OdubanjoDepartment of Biology, Jackson State University, Jackson, MS 39217, USA.
Felicite K NoubissiDepartment of Biology, Jackson State University, Jackson, MS 39217, USA.
Yi-Ming LiuDepartment of Chemistry, Physics, and Atmospheric Science, Jackson State University, Jackson, MS 39217, USA.ORCID 0000-0002-7760-9565

Funding

Strengthening the Biomedical Data Science Training Program at Jackson State UniversityU54MD015929 · NIMHD · JACKSON STATE UNIVERSITY · PI ANTOINE-LAVIGNE, DONNA A · 2020 to 2024
$12.1M
PCR-free UPLC-MS/MS based quantitative assay of microRNAsR16GM145223 · NIGMS · JACKSON STATE UNIVERSITY · PI YIMING LIU · 2022 to 2026
$729k
NIGMS NIH HHS R16 GM145223NIMHD NIH HHS U54 MD015929
6 · The paper itself

Abstract

MicroRNAs (miRNAs) are key regulators of numerous physiological and pathological processes and hold substantial promise for disease diagnosis and therapeutic evaluation. Although a variety of quantitative miRNA assays have been reported, none are suitable for real-time monitoring of miRNA release from live cells due to prolonged assay times, insufficient sensitivity, or interference from complex sample matrices. Herein, we report a novel amplification-free assay based on hybridization of a single-stranded DNA (ssDNA) probe to the target miRNA and fluorescence readout of the resulting DNA-RNA heteroduplex using the RNA-selective intercalating dye SYBR Green II. The proposed assay enables quantitative detection of target miRNAs with a detection limit of 9.1pM and a linear fluorescence response across a concentration range of 25 to 200pM (R

Indexed as

Amplification-free assayIn-situ monitoringLabel-free fluorescenceLive-cell analysismiRNA releaseSYBR green II

Identifiers

PMID42780876
PMCPMC13599371

What Socratic holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.