Evidence map›Paper›PMID 42787191›Full record

ArticleFrontiers in immunology2026

P2X4 purinergic receptor loss alters macrophage homeostasis, cytokine secretion, and phagocytosis in a myeloid-specific P2X4 purinergic receptor knockout mouse.

Vahinipriya Manoharan, Kennedy M Rains, Tristan R Ibarra, Ashwin Barath Vaidhyalingham, Taylor R Pistone, Praise O Awogbesan, Fernanda Caramella, Caylee D Kelso, Jelani O Jarrett, Melanie Quiñones Llanos and 4 more

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Article in Frontiers in immunology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors.

Vahinipriya ManoharanDepartment of Pathology, Johns Hopkins University School of Medicine, Baltimore, MD, United States.
Kennedy M RainsDepartment of Urology, James Buchanan Brady Urological Institute, Johns Hopkins University School of Medicine, Baltimore, MD, United States.
Tristan R IbarraDepartment of Pathology, Johns Hopkins University School of Medicine, Baltimore, MD, United States.
Ashwin Barath VaidhyalinghamDepartment of Pathology, Johns Hopkins University School of Medicine, Baltimore, MD, United States.
Taylor R PistoneDepartment of Pathology, Johns Hopkins University School of Medicine, Baltimore, MD, United States.
Praise O AwogbesanDepartment of Urology, James Buchanan Brady Urological Institute, Johns Hopkins University School of Medicine, Baltimore, MD, United States.
Fernanda CaramellaDepartment of Pathology, Johns Hopkins University School of Medicine, Baltimore, MD, United States.
Caylee D KelsoDepartment of Pathology, Johns Hopkins University School of Medicine, Baltimore, MD, United States.
Jelani O JarrettDepartment of Pathology, Johns Hopkins University School of Medicine, Baltimore, MD, United States.
Melanie Quiñones LlanosDepartment of Pathology, Johns Hopkins University School of Medicine, Baltimore, MD, United States.
Sophia O Olatunji-RichardBloomberg~Kimmel Institute for Cancer Immunotherapy, Johns Hopkins University School of Medicine, Baltimore, MD, United States.
Rieko MuramatsuDepartment of Molecular Pharmacology, National Institute of Neuroscience, National Center of Neurology and Psychiatry, Tokyo, Japan.
David E SaninBloomberg~Kimmel Institute for Cancer Immunotherapy, Johns Hopkins University School of Medicine, Baltimore, MD, United States.
Janielle P MaynardDepartment of Pathology, Johns Hopkins University School of Medicine, Baltimore, MD, United States.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Introduction: The P2X4 purinergic receptor is robustly expressed on tumor-associated macrophages; however, the role of P2X4 purinergic receptors on macrophages is not fully defined. To investigate the functional role of P2X4 purinergic receptor in macrophages, we generated and characterized a myeloid-specific P2X4 purinergic receptor knockout (P2X4 Methods: Immune profiles were assessed across multiple tissues using multiplex immunohistochemistry and flow cytometry. Bulk RNA sequencing, gene set enrichment analysis (GSEA), and Gene Ontology over-representation analysis (ORA) were performed to determine the transcriptional consequence of P2X4 purinergic receptor loss in macrophages. Array and ELISA analyses of cytokine secretion, phagocytosis assays and Seahorse extracellular flux analysis of oxygen consumption were used to assess the functional role of P2X4 purinergic receptors in bone marrow-derived macrophages. Results: P2X4 purinergic receptor loss in macrophages from male mice resulted in reduced tissue-resident macrophage density in liver, spleen, and peritoneal tissues. In addition, male P2X4 Conclusions: Collectively, these findings demonstrate that macrophage specific P2X4 purinergic receptor signaling regulates macrophage homeostasis, cytokine secretion, phagocytosis, and alters the expression of angiogenesis-associated genes.

Indexed as

CytokinesHomeostasisMacrophagesPhagocytosisReceptors, Purinergic P2X4AnimalsFemaleMaleMiceMice, KnockoutMyeloid CellsCytokinesReceptors, Purinergic P2X4angiogenesiscytokine secretionLysMcre micemacrophagesP2X4 purinergic receptorphagocytosispurinergic signalingtissue-resident macrophages

Identifiers

PMID42787191
PMCPMC13600901

What Socratic holds

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.