Evidence map›Paper›PMID 42789383›Full record

ArticleSTAR protocols2026

Protocol for quantitative image analysis of alizarin red staining to assess osteogenesis in cultured cells.

Franceska Kishta, Tanushree Tripathi, Matthieu Vermeren, Andrew H Baker, Mihaela Crisan

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in STAR protocols, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Franceska KishtaCentre for Cardiovascular Science, Institute for Neuroscience and Cardiovascular Research, University of Edinburgh, Edinburgh EH16 4TJ, UK.
Tanushree TripathiCentre for Regenerative Medicine, Institute for Regeneration and Repair, University of Edinburgh, Edinburgh EH16 4UU, UK.
Matthieu VermerenCentre for Regenerative Medicine, Institute for Regeneration and Repair, University of Edinburgh, Edinburgh EH16 4UU, UK.
Andrew H BakerCentre for Cardiovascular Science, Institute for Neuroscience and Cardiovascular Research, University of Edinburgh, Edinburgh EH16 4TJ, UK; Department of Pathology, CARIM School for Cardiovascular Diseases, Maastricht University, Maastricht 6229ER, the Netherlands.
Mihaela CrisanCentre for Cardiovascular Science, Institute for Neuroscience and Cardiovascular Research, University of Edinburgh, Edinburgh EH16 4TJ, UK; Centre for Regenerative Medicine, Institute for Regeneration and Repair, University of Edinburgh, Edinburgh EH16 4UU, UK. Electronic address: mihaela.crisan@ed.ac.uk.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Endothelial-to-mesenchymal transition (EndMT) generates mesenchymal-like cells with multilineage differentiation potential. We describe steps for inducing EndMT in human venous endothelial cells, directing their differentiation toward an osteogenic lineage, and assessing mineralization using an alizarin red assay. We then detail procedures for quantifying and classifying osteogenic capacity from alizarin red staining using custom image analysis pipelines. This protocol can also be used to quantify alizarin red staining in mouse-derived mesenchymal stem/stromal cells (MSCs) in vitro. For complete details on the use and execution of this protocol, please refer to Kishta et al.

Indexed as

Cell BiologyMicroscopyStem Cells

Identifiers

PMID42789383
PMCPMC13635507

What Socratic holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.