Evidence map›Paper›PMID 42791355›Full record

ArticleNature biotechnology2026

Photolabile oligonucleotides with topological light gradients enable spatially resolved single-cell transcriptomics and epigenomics.

Robert A Piscopio, Alex Chialastri, Chieh Wang, Mikolaj Godzik, Kellie A Heom, Weiyue Wang, Lauren J Li, Neha Saxena, Maxwell Z Wilson, Siddharth S Dey

Abstract read
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In one paragraph

Article in Nature biotechnology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

10 authors.

Robert A Piscopio *Department of Molecular, Cellular, and Developmental Biology, University of California, Santa Barbara, Santa Barbara, CA, USA.ORCID http://orcid.org/0000-0002-4115-2874
Alex Chialastri *Department of Chemical Engineering, University of California, Santa Barbara, Santa Barbara, CA, USA.ORCID http://orcid.org/0000-0002-1741-8361
Chieh Wang *Department of Chemical Engineering, University of California, Santa Barbara, Santa Barbara, CA, USA.
Mikolaj Godzik *Department of Molecular, Cellular, and Developmental Biology, University of California, Santa Barbara, Santa Barbara, CA, USA.ORCID http://orcid.org/0009-0001-0854-4358
Kellie A HeomDepartment of Chemical Engineering, University of California, Santa Barbara, Santa Barbara, CA, USA.
Weiyue WangDepartment of Molecular, Cellular, and Developmental Biology, University of California, Santa Barbara, Santa Barbara, CA, USA.
Lauren J LiDepartment of Molecular, Cellular, and Developmental Biology, University of California, Santa Barbara, Santa Barbara, CA, USA.ORCID http://orcid.org/0009-0003-7769-7336
Neha SaxenaDepartment of Chemical Engineering, University of California, Santa Barbara, Santa Barbara, CA, USA.
Maxwell Z WilsonDepartment of Molecular, Cellular, and Developmental Biology, University of California, Santa Barbara, Santa Barbara, CA, USA. mzw@ucsb.edu.ORCID http://orcid.org/0000-0003-0768-7004
Siddharth S DeyDepartment of Chemical Engineering, University of California, Santa Barbara, Santa Barbara, CA, USA. sdey@ucsb.edu.ORCID http://orcid.org/0000-0002-1161-1654

Funding

A marker-free technology for mapping the epigenome of cell types in mammalian tissuesR01HG011013 · NHGRI · UNIVERSITY OF CALIFORNIA SANTA BARBARA · PI DEY, SIDDHARTH SUBHAS · 2020 to 2024
$1.8M
Understanding DNA methylation reprogramming dynamics during preimplantation development using single-cell sequencingR01HD099517 · NICHD · UNIVERSITY OF CALIFORNIA SANTA BARBARA · PI DEY, SIDDHARTH SUBHAS · 2019 to 2023
$1.4M
National Science Foundation (NSF) 2339849U.S. Department of Health & Human Services | NIH | Eunice Kennedy Shriver National Institute of Child Health and Human Development (NICHD) R01HD099517U.S. Department of Health & Human Services | NIH | National Human Genome Research Institute (NHGRI) R01HG011013
6 · The paper itself

Abstract

We introduce scSTAMP-seq (single-cell spatial transcriptomic and multiomic profiling), a modular light-encoded barcoding strategy that preserves spatial information while leveraging standard single-cell platforms. scSTAMP-seq labels live and fixed cells with cholesterol-conjugated, photocleavable hashtag oligonucleotides, followed by patterned light exposure that 'stamps' spatial coordinates onto individual cells before single-cell multiome sequencing. Here we apply scSTAMP-seq to human embryoids showing how spatial organization and epigenetic states coregulate cellular programs in heterogeneous biological systems.

Identifiers

PMID42791355

What Socratic holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.