Evidence map›Paper›PMID 42811264›Full record

ArticleMolecular plant pathology2026

Genome-Wide Screening and Functional Verification of the Tobacco SGR Family Identify Transcription-Factor-Like NtSGRL as a Negative Regulator of Tomato Yellow Leaf Curl Virus Resistance.

Ming Gu, Kexin Cao, Jiamin Yu, Dongyang Liu, Bin Zhao, Siyu Sun, Zhenhong Zhu, Mengnan An, Zihao Xia, Zhiping Wang and 1 more

Abstract read
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In one paragraph

Article in Molecular plant pathology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0citing papers in PubMed
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Ming GuLiaoning Key Laboratory of Plant Pathology, College of Plant Protection, Shenyang Agricultural University, Shenyang, China.ORCID https://orcid.org/0009-0001-6534-7772
Kexin CaoLiaoning Key Laboratory of Plant Pathology, College of Plant Protection, Shenyang Agricultural University, Shenyang, China.
Jiamin YuTobacco Science Research Institute, China National Tobacco Corporation Sichuan Branch, Chengdu, Sichuan, China.
Dongyang LiuLiangshan Prefecture Branch, China National Tobacco Corporation Sichuan Branch, Chengdu, Sichuan, China.
Bin ZhaoLiaoning Key Laboratory of Plant Pathology, College of Plant Protection, Shenyang Agricultural University, Shenyang, China.
Siyu SunLiaoning Key Laboratory of Plant Pathology, College of Plant Protection, Shenyang Agricultural University, Shenyang, China.
Zhenhong ZhuLiaoning Key Laboratory of Plant Pathology, College of Plant Protection, Shenyang Agricultural University, Shenyang, China.
Mengnan AnLiaoning Key Laboratory of Plant Pathology, College of Plant Protection, Shenyang Agricultural University, Shenyang, China.ORCID https://orcid.org/0000-0002-6187-6984
Zihao XiaLiaoning Key Laboratory of Plant Pathology, College of Plant Protection, Shenyang Agricultural University, Shenyang, China.ORCID https://orcid.org/0000-0001-7905-106X
Zhiping WangLiaoning Key Laboratory of Plant Pathology, College of Plant Protection, Shenyang Agricultural University, Shenyang, China.
Yuanhua WuLiaoning Key Laboratory of Plant Pathology, College of Plant Protection, Shenyang Agricultural University, Shenyang, China.ORCID https://orcid.org/0000-0001-6121-7033

Funding

Zihao Xia SCYC202605
6 · The paper itself

Abstract

Viral infection often induces chlorosis, a symptom closely associated with chlorophyll (Chl) degradation. STAY-GREEN (SGR) proteins are key regulators of Chl breakdown, but their roles in plant virus resistance remain poorly understood. In this study, we identified six NtSGR family genes in Nicotiana tabacum and functionally characterized NtSGRL1 during tomato yellow leaf curl virus (TYLCV) infection. TYLCV drastically upregulates NtSGRL1 (149-fold at 4 days post-inoculation [dpi] vs. control), suggesting its involvement in the tobacco-TYLCV interaction. Subcellular localization revealed that NtSGRL1 is targeted to both the nucleus and chloroplast, and yeast assays confirmed its transcriptional activation activity, indicating potential dual functions. TYLCV accumulation was reduced by 1995-fold in Ntsgrl1 mutants relative to wild-type (WT) plants at 10 dpi. Further assays with tomato spotted wilt virus (TSWV), potato virus Y (PVY) and tobacco mosaic virus (TMV) showed that viral accumulation in Ntsgrl1 mutants was reduced by 62%-99%, suggesting that NtSGRL1 may affect resistance to additional tested viruses. Transcriptomics revealed 1470 differentially expressed genes in TYLCV-infected Ntsgrl1 plants enriched in MAPK signalling and phenylpropanoid biosynthesis pathways. Virus-induced gene silencing (VIGS)-based validation further supported the involvement of these pathways in TYLCV resistance. Overall, this study identifies NtSGRL1 as a critical regulator of antiviral resistance in tobacco and reveals its dual functional roles as both a Chl-related enzyme and a transcriptional regulator. These findings provide new insights into plant antiviral mechanisms and potential genetic resources for breeding virus-resistant crops.

Indexed as

BegomovirusDisease ResistanceNicotianaPlant DiseasesPlant ProteinsTranscription FactorsGene Expression Regulation, PlantPlant ProteinsTranscription Factorsbroad‐spectrum antiviral activitydual‐function proteinnegative regulationSTAY‐GREEN (SGR)tomato yellow leaf curl virus (TYLCV)

Identifiers

PMID42811264
PMCPMC13624059

What Socratic holds

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.