Evidence map›Paper›PMID 42811372›Full record

ArticleVirology journal2026

Cell culture medium influences RSV G protein glycosylation and recognition by monoclonal antibodies.

Nele Engling, Marie-Christin Knittler, Rajarshee Tagore, Martin Ludlow, Albert D M E Osterhaus, Guus F Rimmelzwaan, Robert Meineke

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Virology journal, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Nele EnglingResearch Center for Emerging Infections and Zoonoses (RIZ), University of Veterinary Medicine Hannover, Hannover, Germany.
Marie-Christin KnittlerResearch Center for Emerging Infections and Zoonoses (RIZ), University of Veterinary Medicine Hannover, Hannover, Germany.
Rajarshee TagoreResearch Center for Emerging Infections and Zoonoses (RIZ), University of Veterinary Medicine Hannover, Hannover, Germany.
Martin LudlowResearch Center for Emerging Infections and Zoonoses (RIZ), University of Veterinary Medicine Hannover, Hannover, Germany.
Albert D M E OsterhausResearch Center for Emerging Infections and Zoonoses (RIZ), University of Veterinary Medicine Hannover, Hannover, Germany.
Guus F RimmelzwaanResearch Center for Emerging Infections and Zoonoses (RIZ), University of Veterinary Medicine Hannover, Hannover, Germany.
Robert MeinekeResearch Center for Emerging Infections and Zoonoses (RIZ), University of Veterinary Medicine Hannover, Hannover, Germany. robert.meineke@tiho-hannover.de.ORCID https://orcid.org/0000-0003-4191-8021

Funding

Deutsche Forschungsgemeinschaft 398066876/GRK 2485/2
6 · The paper itself

Abstract

Respiratory syncytial virus (RSV) glycoprotein G is heavily glycosylated and of variable size following virus growth in transformed cell lines or primary cells. However, it is unknown if the cell culture medium can influence the patterns of glycosylation. Therefore, we tested whether the choice of culture medium can affect the glycosylation of RSV G protein, viral infectivity and antibody recognition. The RSV G ectodomain was produced in HEK293T cells cultured in three different media. Marked differences in molecular weight were observed that were abolished in glycosylation-deficient knockout cells. RSV produced in A549 cells cultured in the same media also differed in relative G protein signals in virus stocks and virus spread. Higher apparent G protein glycosylation was associated with lower binding signals of human F protein-specific monoclonal antibodies to RSV-infected cells. These data show that the cell culture medium can influence biological properties of RSV preparations with consequences for antibody recognition and viral spread.

Indexed as

Antibodies, MonoclonalAntibodies, ViralCulture MediaRespiratory Syncytial Virus, HumanViral Fusion ProteinsA549 CellsGlycosylationHEK293 CellsHumansAntibodies, MonoclonalAntibodies, ViralCulture MediaG glycoprotein, Respiratory syncytial virusViral Fusion ProteinsCulture mediumGlycoproteinGlycosylationMonoclonal antibodyRespiratory syncytial virus

Identifiers

PMID42811372
PMCPMC13621597

What Socratic holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.