Lyophilized rumen fluid from goats fed different roughage-based diets: fermentation characteristics, microbial communities, and post-reconstitution

Meila Na, Wenliang Guo, Ran Zhang, Haidong Du, Kenan Li, Jing Zhang, Renhua Na

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Article in Frontiers in microbiology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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5 · Who and what money

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7 authors.

Meila NaCollege of Animal Science, Inner Mongolia Agricultural University, Hohhot, China.

Wenliang GuoCollege of Food and Agriculture, Hetao College, Bayannur, China.

Ran ZhangCollege of Animal Science, Inner Mongolia Agricultural University, Hohhot, China.

Haidong DuCollege of Animal Science and Technology, Inner Mongolia Minzu University, Tongliao, China.

Kenan LiInstitute of Grassland Research of Chinese Academy of Agricultural Sciences, Hohhot, China.

Jing ZhangCollege of Animal Science, Inner Mongolia Agricultural University, Hohhot, China.

Renhua NaCollege of Animal Science, Inner Mongolia Agricultural University, Hohhot, China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Introduction: Fresh rumen fluid is a promising donor material for rumen fluid transplantation (RFT), but poor storage stability and batch variability limit its use. Methods: In this study, lyophilized rumen fluid samples from goats fed alfalfa hay or corn stover diets were designated AHP and CSP, respectively. For the Results: Lyophilization yields were 6.29 and 6.38%, and culturable carbohydrate-utilizing bacterial counts were 3.79 × 10⁷ and 3.93 × 10⁷ CFU/g, respectively, with no significant differences between AHP and CSP. AHP contained higher propionate and valerate concentrations ( Discussion: Overall, the roughage source in the donor diets influenced the fermentation characteristics and microbial composition of lyophilized rumen fluid. After reconstitution, both inocula fermented exogenous substrate and exhibited different fermentation profiles, supporting their potential as storable inocula for standardization in RFT.

Indexed as

fermentation parametersin vitro fermentationlyophilized rumen fluidroughage sourcerumen fluid transplantationrumen microbiota

Identifiers

PMID42812573

DOI10.3389/fmicb.2026.1905439

PMCPMC13619837

What Socratic holds

Textmetadata

Read underepoch 390

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.