Evidence map›Paper›PMID 42814352›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2027

Live Imaging of Grass Roots Growth Dynamics Using Microfluidics.

Lev Levenets, Dominik Kralík, Vasyl Brykov, Matyáš Fendrych

Abstract read
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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2027. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Lev LevenetsInstitute of Experimental Botany of the Czech Academy of Sciences, Prague, Czech Republic.
Dominik KralíkInstitute of Experimental Botany of the Czech Academy of Sciences, Prague, Czech Republic.
Vasyl BrykovInstitute of Experimental Botany of the Czech Academy of Sciences, Prague, Czech Republic.
Matyáš FendrychInstitute of Experimental Botany of the Czech Academy of Sciences, Prague, Czech Republic. fendrych.m@ueb.cas.cz.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Microfluidic tools enable manipulation of microliter-scale volumes within a stable microenvironment. Microfluidics has been widely adopted in various biological fields for, among other applications, controlled delivery of sensory, chemical, and mechanical stimuli to the observed organisms. In this chapter, we describe the design and usage of a custom-made double-layered PDMS microfluidic device, named BUGchip, developed for the purpose of studying plant root growth and metabolic responses in rapidly changing conditions with a high time-scale resolution. We demonstrate the utility of this device by a showcase experiment with a hyperosmotic treatment of Brachypodium distachyon roots, in which changes in growth rate and relative pH across the root surface in response to the treatment can be observed within minutes.

Indexed as

BrachypodiumMicrofluidic Analytical TechniquesMicrofluidicsPlant RootsHydrogen-Ion ConcentrationLab-On-A-Chip DevicesBrachypodium distachyonLive imagingMicrofluidicsMicroscopyNoninvasive treatmentRoot growth

Identifiers

PMID42814352

What Socratic holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.