ArticleReproduction in domestic animals = Zuchthygiene2026
Silver Nanoparticles in Donkey Semen Stored at 17°C: Effect on Sperm Parameters and Bacterial Growth.
Article in Reproduction in domestic animals = Zuchthygiene, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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13 authors.
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Abstract
The aim of this study was to evaluate the effect of silver nanoparticles (AgNPs) on donkey sperm parameters and their antimicrobial efficacy on bacteria isolated in the presence of antibiotics. Nine donkey ejaculates were collected and stored in a skim-milk extender at different AgNPs concentrations (0, 0.01, 0.8, 1.6 and 3.12 mg/mL) at 5°C or 17°C. Sperm parameters, including total and progressive motility (CASA), plasma membrane integrity (PMI) and reactive oxygen species (rROS) production, were evaluated at 0, 24 and 48 h of storage. Additionally, bacteria isolated from donkey semen extended with antibiotics were exposed to AgNPs to determine the minimum inhibitory/bactericidal concentrations (MIC/MBC). Sperm motility parameters were higher at 17°C than 5°C after 48 h. AgNPs showed a dose- and time-dependent cytotoxic effect, with concentrations ≥ 0.8 mg/mL significantly reducing motility, membrane integrity and increasing rROS after 24 h. AgNPs exhibited bactericidal activity at concentrations between 1.56 and ≥ 25 mg/mL. In conclusion, while liquid storage at 17°C is an effective strategy for preserving donkey semen, the use of AgNPs as a standalone antimicrobial is currently limited by their cytotoxicity at effective bactericidal doses.
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