DNA Nanotechnology in the Undergraduate Laboratory: Isothermal Assembly and Quantification of DNA Nanostructures.

Hannah Talbot, Sarah Youssef, Arun Richard Chandrasekaran

Abstract read

In one paragraph

Article in JACS Au, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it

0cells of the map it votes in

0citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Hannah TalbotDepartment of Nanoscale Science and Engineering, University at Albany, State University of New York, Albany, New York 12222, United States.

Sarah YoussefDepartment of Nanoscale Science and Engineering, University at Albany, State University of New York, Albany, New York 12222, United States.

Arun Richard ChandrasekaranDepartment of Nanoscale Science and Engineering, University at Albany, State University of New York, Albany, New York 12222, United States.ORCID https://orcid.org/0000-0001-6757-5464

Funding

RNA Science and Technology in Health and DiseaseT32GM132066 · NIGMS · STATE UNIVERSITY OF NEW YORK AT ALBANY · PI Thomas J Begley · 2019 to 2026

$1.8M

Programmable DNA Nanostructures as Biomedical and Structural ScaffoldsR35GM150672 · NIGMS · STATE UNIVERSITY OF NEW YORK AT ALBANY · PI Arun Richard Chandrasekaran · 2023 to 2026

$1.7M

NIGMS NIH HHS R35 GM150672NIGMS NIH HHS T32 GM132066

6 · The paper itself

Abstract

DNA nanotechnology has proven to have strong potential in several applications. This technology relies on the programmable nature of DNA self-assembly to build structures and motifs with a high level of precision. Most assembly techniques rely on a temperature gradient that might not be suitable for all applications, especially those involving other biomolecules. Here, we introduce the isothermal assembly concept that relies on a single temperature to assemble DNA nanostructures. This simple concept can be easily implemented in undergraduate-level training. We present a simple experimental setup for introducing undergraduate students to DNA nanotechnology and learning characterization techniques such as gel electrophoresis. We believe that basic DNA nanotechnology protocols can boost the level of undergraduate research and enrich the interdisciplinary skill set of students, especially those working in molecular biology and biochemistry laboratories.

Indexed as

BiochemistryDNA nanotechnologyElectrophoresisHands-on learning/manipulativesNanotechnologyNucleic acids/DNA/RNAUndergraduate researchUpper-division undergraduate

Identifiers

PMID42819240

DOI10.1021/jacsau.6c01181

PMCPMC13625532

What Socratic holds

Textmetadata

Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.