ArticleFrontiers in veterinary science2026
Development and validation of a multiplex TaqMan real-time quantitative PCR assay for the simultaneous detection of six major bacterial respiratory pathogens in goats.
Article in Frontiers in veterinary science, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
Background: Respiratory disease caused by bacterial and mycoplasmal pathogens is an important threat to goat health, and mixed infections complicate etiological diagnosis. Rapid multiplex detection is therefore valuable for clinical investigation and surveillance. Methods: Two triplex TaqMan real-time quantitative PCR (qPCR) panels were established for the simultaneous detection of six major caprine respiratory pathogens. Panel I targeted Results: No amplification was observed for the tested non-target organisms. The limits of detection were 1.73 × 10 Conclusion: The multiplex qPCR assay developed in this study provides a rapid and specific tool for the simultaneous detection of six major respiratory pathogens in goats, with potential utility for clinical diagnosis and epidemiological surveillance.
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