ArticleUltrasonics sonochemistry2026
Ultrasound-driven ternary conjugation reshapes protein-lipid interfaces and volatile profiles in high-protein egg milk systems.
Article in Ultrasonics sonochemistry, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
Heat-induced aggregation, sulfurous off-flavors, and oxidative instability pose challenges for the use of egg white proteins (EWPs) in high-protein beverages. In this study, a ternary conjugated system comprising EWP, milk casein, and xylitol (EMX) was incorporated into skimmed milk powder (SMP) and whole-fat milk powder (WFM) beverages, which were subsequently treated with ultrasound (US; 20 kHz, 300 W). Physicochemical properties, oxidative stability, volatile compounds, in vitro digestion, and storage stability over 30 days at 4 °C were monitored. The results showed that EMX conjugated system exhibited higher solubility (91.04 % vs. 88.54 %) and a 2.4-fold reduction in turbidity compared with skimmed beverages. Ultrasound further decreased particle size and increased molecular flexibility in whole-fat formulations. Additionally, EMX showed the greatest thermal stability (Td 114.17 °C; ΔHd 179.3 J/g), while US treatment improved Td from 102.21 °C to 113.23 °C in SMP. US-treated whole-fat beverages demonstrated lower TBARS values during days 0-14 but higher values than their untreated counterparts on day 30. Protein carbonyl levels remained lowest in US-treated skimmed beverages throughout storage. Altogether, the US increased digestibility, while mitigating dipropyl disulfide and 2-furanmethanethiol sulfurous off-flavors. In conclusion, US-assisted EMX ternary conjugation offers a promising approach for developing the functionality and sensory qualities of protein-enriched dairy model systems.
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